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ID   PFLAG1     preliminary; circular DNA; SYN; 51 BP.
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AC   IG5021;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pFLAG-1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pFLAG-1 from polypeptide marker]
RC   pIXY8 from IL-2 gene & pFLAG-1
RC   pRL6-6-87 from pIN-III-ompA3 & GM-CSF gene & oligo
RA   Hopp T.P., Prickett K.S., Price V.L., Libby R.T., March C.J.,
RA   Cerretti D.P., Urdal D.L., Conlon P.J.;
RT   "A short polypeptide marker sequence useful for recombinant protein
RT   identification and purification";
RL   Biotechnology 6:1204-1210(1988).
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RN   [2]
RC   pFLAG-TNFalpha from pFLAG-1
RA   Su X., Prestwood A.K., McGraw R.A.;
RT   "Production of recombinant porcine tumor necrosis factor alpha in a
RT   novel E. coli expression system";
RL   Biotechniques 13:756-762(1992).
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RN   [3]
RC   plasmid from pBR322 & yeast 2 micron & TRP1 gene & ADH2 promoter
RC   palphaADH2 from plasmid & alpha factor promoter
RA   Price V.L., Mochizuki D., March C.J., Cosman D., Deeley M.C.,
RA   Klinke R., Clevenger W., Gillis S., Baker P., Urdal D.L.;
RT   "Expression, purification and characterization of recombinant
RT   murine granulocycte-macrophage colony stimulating factor and bovine
RT   interleukin-2 from yeast";
RL   Gene 55:287-293(1987).
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CC   FLAG peptide is cleaved away with enterokinase.
CC   NM (pFLAG-1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (IBI)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. yeast 2 micron
FT                   3. yeast, TRP1 gene
FT                   4. ADH2 promoter or alpha factor promoter
FT                   5. FLAG peptide sequence
FT                   -> pFLAG-1"
FT   promoter        0..0
FT                   /note="PRO E. coli OmpA/FLAG peptide"
FT   misc_feature    1..9
FT                   /note="E. coli OmpA signal sequence"
FT   misc_feature    10..30
FT                   /note="FLAG peptide"
FT   misc_binding    31..51
FT                   /note="MCS HindIII-EcoRI-XbaI"
FT   misc_binding    31..36
FT                   /note="SIT HindIII"
FT   misc_binding    37..42
FT                   /note="SIT EcoRI"
FT   misc_binding    43..48
FT                   /note="SIT XbaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 51 BP; 17 A; 11 C; 13 G; 10 T; 0 other;
     gcgcaagctg actacaagga cgacgatgac aagcttgaat tctctagaga t
//