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ID   PFMDVYEPPC preliminary; circular DNA; SYN; 400 BP.
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AC   M35873;
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DT   15-SEP-1990 (Rel. 5, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Vertebrate virus vector pFMDV-YEP-polyC - incomplete, FMDV/SP6.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-400
RC   pFMDV-L from pSP64 & FMDV strain 01K
RC   pFMDV-S from pSP64 & FMDV strain 01K
RC   pFMDV-ff from pFMDV-L & pFMDV-S
RC   pFMDV-YEP from YEp51 & pFMDV-ff
RC   pFMDV-YEP-polyC from pFMDV-YEP & oligo
RA   Zibert A., Maass G., Strebel K., Falk M.M., Beck E.;
RT   "Infectious foot-and-mouth disease virus derived from a cloned
RT   full-length cDNA";
RL   J. Virol. 64:2467-2473(1990).
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RN   [2]
RC   YEp51 from YEp series
RA   Broach J.R., Li Y., Wu C., Jayram M.;
RT   "Vectors for high-level, inducible expression of cloned genes in
RT   yeast";
RL   Experimental Manipulation of Gene Expression 0:83-117(1983).
RL   Inouye M., ed., Academic Press, New York.
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CC   FMDV = foot-and-mouth disease virus (strain O1K).
CC   NCBI gi: 209575
CC   NM (pFMDV-YEP-polyC)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (foot-and-mouth disease virus O1K)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP64 SmaI
FT                   HpaI linker
FT                   2. FMDV strain 01K HpaI-HpaI
FT                   -> pFMDV-L
FT                   1. pSP64 EcoRI
FT                   2. FMDV strain 01K EcoRI-EcoRI
FT                   -> pFMDV-S
FT                   1. pFMDV-L NheI-PvuI 8700bp, L fragment
FT                   2. pFMDV-S NheI-PvuI 2700bp, S fragment/Sp6
FT                   -> pFMDV-ff 11400bp
FT                   1. YEp51 remove BamHI-HindIII, MCS
FT                   Klenow fill in:Klenow fill in
FT                   2. pFMDV-ff HpaI-NheI 9000bp
FT                   Klenow fill in:Klenow fill in
FT                   -> pFMDV-YEP
FT                   1. pFMDV-YEP NheI
FT                   Klenow fill in:Klenow fill in
FT                   2. oligo polyC
FT                   -> pFMDV-YEP-polyC"
FT   misc_signal     1..17
FT                   /note="PRO bacteriophage Sp6"
FT   misc_RNA        18..396
FT                   /note="RNA"
FT   misc_recomb     29..30
FT                   /note="plasmid DNA end/foot-and-mouth disease DNA
FT                   start"
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SQ   Sequence 400 BP; 68 A; 122 C; 112 G; 98 T; 0 other;
     atttaggtga cactatagaa tacaagctct tgaaaggggg cattagggtc tcacccctag
     taagccaacg acagtccctg cgttgcactc cacacttacg ttgtacacac gcgggacccg
     atgggctatc gttcacccac ctacagctgg actcacggcg ccgcgtggcc atttagctgg
     attgtgcgga cgaacacgct tgcgcacctc gcgtgaccgg ttagtactct taccactctc
     cgcctacttg gtcgttagcg ctgtcttggg cattcctgtg ggggccgttc gacgctccac
     gggaactctc ctgtgtgaca tctacggtga tggggccgtt tcgcgtgggc tggtcgtttg
     gactgcttcg gctgtcaccc ggcgcccgcc tttcagctag
//