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ID   PFRSVX     preliminary; circular DNA; SYN; 389 BP.
XX
AC   M18804;
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DT   09-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Mouse Friend leukemia retrovirus vector Fr-MuLV pFR-SV(X) - incompl.
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KW   cloning vector; retrovirus; leukemia; retroviral vector;
KW   Friend murine leukemia virus.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-389
RC   plasmid from Fr-MuLV enhancer & MMLV
RC   pFR-SV(X) from pZIP-SV(x) & plasmid
RC   plasmid2 from pFR-SV(X) & pcDV1
RC   from plasmid2 & pZIP-SV(x) & Fr-MuLV LTR
RA   Holland C.A., Anklesaria P., Sakakeeny M.A., Greenberger J.S.;
RT   "Enhancer sequences of a retroviral vector determine expression of a
RT   gene in multipotent hematopoietic progenitors and committed
RT   erythroid cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:8662-8666(1987).
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CC   NCBI gi: 209260
CC   NM (pFR-SVX)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Moloney murine leukemia virus)(Friend murine leukemia virus)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. MMLV ClaI-Sau3A, p15E gene to LTR
FT                   2. Fr-MuLV Sau3A-KpnI, LTR to LTR/enhancer
FT                   -> fragment [ClaI-KpnI]
FT                   1. pZIPneoSV(x)1 remove ClaI-KpnI, neo gene
FT                   2. fragment ClaI-KpnI, Fr-MuLV LTR
FT                   -> pFR-SV(X) [1 LTR]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 389 BP; 90 A; 124 C; 97 G; 78 T; 0 other;
     gatcaaggtc aggtacacga aaacagctaa cgttgggcca aacaggatat ctgtggtaag
     cagtttcggc cccggcccgg ggccaagaac agatacgctg ggccaaacag gatatctgtg
     gtaagcagtt tcggcccggt cggccccggc ccgaggccaa gaacggatgg tccccagata
     tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     ctgaaatgac cctgtgcctt atttgaatta accaatcagc ccgcttctcg cttctgttcg
     cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     cctccgatag actgagtcgc ccgggtacc
//