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ID   PFTB281    preliminary; circular DNA; SYN; 5900 BP.
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AC   K02661;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pFTB281 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pFTB232, pFTB233, pFTB239 from pUB110 & B.amyloliquefaciens V trpC
RC   pFTB255 from pFTB233
RC   pFTB275 from pFTB255
RC   pFTB281 from M13mp7 & pFTB275
RC   pFTB303, pFTB305, pFTB306, pFTB308, pFTB313 from pFTB281 & pE194
RA   Yoshimura K., Uemura J., Seki T., Oshima Y.;
RT   "Construction of a promoter-probe vector for a Bacillus subtilis
RT   host by using the trpD+ gene of Bacillus amyloliquefaciens";
RL   J. Bacteriol. 159:905-912(1984).
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CC   new sequence. in GenBank.
CC   NM (pFTB281)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13mp7)(pUB110)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUB110 EcoRI-XbaI, kan gene
FT                   2. B.amyloliquefaciens V  EcoRI-XbaI, trpC
FT                   -> pFTB233
FT                   1. pFTB233 remove small EcoRI-EcoRI
FT                   -> pFTB255 [trpC]
FT                   1. pFTB255 remove PvuII-PvuII 1500bp, trp promoter
FT                   -> pFTB275 [unique EcoRI in trpD]
FT                   1. M13mp7 EcoRI-EcoRI 42bp
FT                   2. pFTB275 EcoRI
FT                   -> pFTB281 5900bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BamHI-SalI-PstI-SalI-BamHI-EcoRI"
FT   CDS             0..0
FT                   /note="GEN E. coli trpD gene"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   CDS             0..0
FT                   /note="GEN E. coli trpC gene"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
FT   CDS             0..0
FT                   /note="ANT E. coli kan gene"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
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SQ   Sequence 48 BP; 9 A; 15 C; 15 G; 9 T; 0 other;
     gaattccccg gatccgtcga cctgcaggtc gacggatccg gggaattc
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