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ID   PFX4       preliminary; circular DNA; SYN; 42 BP.
AC   IG5050;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pFX4 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pFX2 from pFX1
RC   pFX3 from pFX2 & pUBS
RC   pFX4 from pFX2 & pNM480
RC   pFX5 from pFX2 & pNM481
RC   pFX6 from pFX2 & pNM482
RC   pFX401, pFX501, pFX601 from pFX series & pDI21
RA   Xu F.F., Pearce L.E., Yu P.L.;
RT   "Construction of a family of lactococcal vectors for gene cloning
RT   and translational fusion";
RL   FEMS Microbiol. Lett. 77:55-60(1991).
RN   [2]
RC   [lambda NM1149 from lambda]
RC   from pDI21 & lambda NM1149
RC   pDI25 from L.lactis
RC   pFX1 from pDI25 & pC194
RC   pFX101 from pFX1 & pDI21
RA   Xu F.F., Pearce L.E., Yu P.L.;
RT   "Molecular cloning and expression of a proteinase gene from
RT   Lactococcus lactis subsp. cremoris H2 and construction of a new
RT   lactococcal vector pFX1";
RL   Arch. Microbiol. 154:99-104(1990).
RN   [3]
RC   pUBS from pUC19 & T3 & T7
RA   Forster R.L.S.;
RT   ;
RL   Unpublished (1991).
RN   [4]
RC   pDI21 from Lactococcus lactis cremoris H2 proteinase gene
RA   Yu P.L., Appleby R.D., Pritchard G.G., Limsowtin G.K.Y.;
RT   "Restriction mapping and localization of the lactose-metabolizing
RT   genes of Streptococcus cremoris pDI-21";
RL   Appl. Microbiol. Biotechnol. 30:71-74(1989).
RN   [5]
RC   pDI21 or pJI70 from S.sanguis/E.coli
RA   Inamine J.M., Lee L.N., LeBlanc D.J.;
RT   "Molecular and genetic characterization of lactose-metabolic genes
RT   of Streptococcus cremoris";
RL   J. Bacteriol. 167:855-862(1986).
RN   [6]
RC   pLP712 from proteinase gene
RC   pSK111 from proteinase gene
RC   pWV05 from proteinase gene
RA   De Vos V.M.;
RT   "Gene cloning and expression in lactic streptococci";
RL   FEMS Microb. Rev. 46:281-295(1987).
RN   [7]
RC   pDI21 or pJI70 from L.lactis
RA   Davey G.P., Crow V.L., Pearce L.E.;
RT   "Enzyme analysis of Lac+ transconjugants of Streptococcus cremoris";
RL   N.Z. J. Dairy Sci. Technol. 19:183-188(1984).
CC   not original sequence. MCS.
CC   NM (pFX4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pFX2)(pNM480)
CC   BR (pFX5)(pFX6)
CC   OF (pFX401)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. L.lactis
FT                   -> pDI25 2600bp
FT                   1. pDI25 2600bp
FT                   2. pC194 2910bp, cat gene
FT                   -> pFX1 5500bp
FT                   1. pFX1 remove ClaI-ClaI 1900bp,pC194 616..2284/3600bp
FT                   -> pFX2 3600bp
FT                   1. pFX2 ClaI 3600bp
FT                   fill in
FT                   2. pNM480 EcoRI-DraI 3100bp
FT                   fill in:fill in
FT                   -> pFX4 6700bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 42 BP; 7 A; 16 C; 11 G; 8 T; 0 other;
     gaattcccgg ggatccgtcg acctgcagcc aagcttgctc cc