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ID   PFX5       preliminary; circular DNA; SYN; 43 BP.
XX
AC   IG5051;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pFX5 - incomplete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pFX2 from pFX1
RC   pFX3 from pFX2 & pUBS
RC   pFX4 from pFX2 & pNM480
RC   pFX5 from pFX2 & pNM481
RC   pFX6 from pFX2 & pNM482
RC   pDI-21 from Lactococcus
RC   pFX401, pFX501, pFX601 from pFX series & pDI-21
RA   Xu F.F., Pearce L.E., Yu P.L.;
RT   "Construction of a family of lactococcal vectors for gene cloning
RT   and translational fusion";
RL   FEMS Microbiol. Lett. 77:55-60(1991).
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CC   not original sequence. MCS.
CC   NM (pFX5)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pFX2)(pNM481)
CC   BR (pFX4)(pFX6)
CC   OF (pFX501)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. L.lactis
FT                   -> pDI25 2600bp
FT                   1. pDI25 2600bp
FT                   2. pC194 2910bp, cat gene
FT                   -> pFX1 5500bp
FT                   1. pFX1 remove ClaI-ClaI 1900bp,pC194 616..2284/3600bp
FT                   -> pFX2 3600bp
FT                   1. pFX2 ClaI 3600bp
FT                   fill in
FT                   2. pNM481 EcoRI-DraI 3100bp
FT                   fill in:fill in
FT                   -> pFX5 6700bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 43 BP; 8 A; 16 C; 11 G; 8 T; 0 other;
     gaattcccgg ggatccgtcg acctgcagcc aagcttcgat ccc
//