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ID   PG1        preliminary; circular DNA; SYN; 3600 BP.
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AC   M30890; M29847; ATCC37305;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pG1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pTRP56 from YRp7 & TRP1 terminator
RC   pA1 from pUC8 & ADC1 gene promoter
RC   pG1 from pUC8 & GAL1 gene promoter
RC   pTRP57 from pTRP56 & CEN3
RC   pcD-Y from pTRP56 & pA1 or pG1
RC   pGAL100 series from pcD-Y
RA   Miyajima A., Nakayama N., Miyajima I., Arai N., Okayama H., Arai K.;
RT   "Analysis of full-length cDNA clones carrying GAL1 gene of
RT   Saccharomyces cerevisiae: a model system for cDNA expression";
RL   Nucleic Acids Res. 12:6397-6414(1984).
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RN   [2]
RC   pG-1 from pUC18 & GPD prom & PGK term & TRP1 gene & yeast 2 micron ori
RC   pG-2 from pG-1
RC   pG-3 from pG-1 & linker
RC   pG-N795 from pG-1 & N795, rat glucocorticoid receptor
RC   pSD-N795 from pLGSD5 & N795, rat glucocorticoid receptor
RC   pG-D from pGPD-556a & pUC18
RA   Schena M., Picard D., Yamamoto K.R.;
RT   "Vectors for constitutive and inducible gene expression in yeast";
RL   Meth. Enzymol. 194:389-398(1991).
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RN   [3]
RC   GMCS from MTV promoter/GRE & cat gene & MoMSV LTR
RA   DeFranco D., Yamamoto K.R.;
RT   "Two different factors act separately or together to specify
RT   functionally distinct activities at a single transcriptional
RT   enhancer";
RL   Mol. Cell. Biol. 6:993-1001(1986).
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RN   [4]
RC   PPCV from SV40 enhancer & cat gene & cow prolactin promoter
RA   Sakai D.D., Helms S., Carlstedt-Duke J., Gustafsson J.A.,
RA   Rottman F.M., Yamamoto K.R.;
RT   "Hormone-mediated repression of transcription: a negative
RT   glucocoritocid response elementr from the bovine prolactin gene";
RL   Genes Dev. 2:1144-1154(1988).
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CC   This was constructed by inserting the yeast GAL1 promoter into pUC8.
CC   The promoter contains the regulatory sequence and the transcription
CC   initiation site, but not the initiation codon.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pG1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MC1061)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC18)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 remove BamHI-PstI, MCS
FT                   2. yeast BamHI-PstI, GAL1 gene promoter
FT                   -> pG1 3600bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO Saccharomyces GAL1 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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