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ID   PGA437     preliminary; circular DNA; SYN; 18299 BP.
AC   ATCC37350;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Higher plants/Agrobacterium/E.coli plasmid vector pGA437 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pETW3 from pFH33
RC   pHoHo1 from pETW3 & pGA300
RC   pSShe from pACYC184 & RSF2124
RC   [pVCK102 from pVCK series]
RC   pVCK261, pVCK2 series from pTiA6 & pVCK102
RC   pSM series from pVCK261
RA   Stachel S.E., An G., Flores C., Nester E.W.;
RT   "A Tn3 lacZ transposon for the random generation of
RT   beta-galactosidase gene fusions: application to the analysis of
RT   gene expression in Agrobacterium";
RL   EMBO J. 4:891-898(1985).
RN   [2]
RC   pT37H23 from pTiT37 & Tn5, neo
RC   pGA342 from pAG60
RC   pGA355 from pGA342 & pT37H23
RC   pGA355-147 from pGA355
RC   pGA428 from pT37H23
RC   pGA430 from pGA355-147 & pGA428
RC   pGA435 from pTiT37 & pGA430
RC   pGA437 from pGA435 & pTJS75
RC   pGA446 from pGA435
RC   pGA451 from pTJS75
RC   pGA452 from MUA-10
RC   pGA469, pGA470 from pGA446 & pGA451
RC   pGA471 from pGA469 & pGA452
RC   pGA472 from pGA470 & pGA452
RC   pGA482 from pHC79 & pTJS75 & pAL4404
RC   NOTE: this reference is incorrect for ATCC37350; see [1] above
RA   An G., Watson B.D., Stachel S.E., Gordon M.P., Nester E.W.;
RT   "New cloning vehicles for transformation of higher plants";
RL   EMBO J. 4:277-284(1985).
RN   [3]
RC   pTJS75 from RK2 & tet
RA   Helinski D.;
RT   ;
RL   Unpublished (1985).
RN   [4]
RC   pGA254 from pSV2-gpt & pBR322
RC   pGA291 from pGA164 & pSV2-gpt
RC   pGA293 from pGA291
RC   pGA296 from pGA293
RC   pGA300 from pGA291 & linker
RC   pGA307 from pGA300 & pGA254
RA   An G., Hidaka K., Siminovitch L.;
RT   "Expression of bacterial beta-galactosidase in animal cells";
RL   Mol. Cell. Biol. 2:1628-1632(1982).
RN   [5]
RC   pJB4J1 from Tn5
RC   from pTiA6 NC & pJB4J1, Tn5
RC   from pTiB6 806 & pJB4J1, Tn5
RA   Garfinkel D.J., Nester E.W.;
RT   "Agrobacterium tumefaciens mutants affected in crown gall
RT   tumorigenesis and octopine catabolism";
RL   J. Bacteriol. 144:732-743(1980).
RN   [6]
RC   lambda 21-10 from lambda drifd18
RC   pGA22 from pACYC177 & pACYC184
RC   pGA24 from pGA22
RC   pGA26 from pGA24 & lambda 21-10
RC   pGA39 from pGA26
RC   pGA36 from pGA26
RC   pGA43 from pGA36 & pNF1310
RC   pGA46 from pGA43
RC   pGA44 from pGA43
RA   An G., Friesen D.;
RT   "Plasmid vehicles for direct cloning of Escherichia coli
RT   promoters";
RL   J. Bacteriol. 140:400-407(1979).
RN   [7]
RC   pNF1310 from E.coli ribosomal protein
RA   Fiil N.P., Bendiak D., Collins J., Friesen J.D.;
RT   "Expression of Escherichia coli ribosomal protein and RNA
RT   polymerase genes cloned on plasmids";
RL   Mol. Gen. Genet. 173:39-50(1979).
RN   [8]
RC   pTiB6S3::RP4 from pTiB6S3 & RP4
RC   from pTiB6S3::RP4 & Tn7
RA   Hernalsteens J.P., De Block M., Van Montagu M., Schell J.;
RT   "Mutagenesis by insertion of the drug resistance transposon Tn7
RT   applied to the Ti-plasmid of Agrobacterium tumefaciens";
RL   Plasmid 1:218-225(1978).
RN   [9]
RC   from pTiACH5 & Tn904
RC   from pTiACH5 & Tn1831
RA   Klapwijk P.M., Van Breukelen J., Korevaar K., Oooms G.,
RA   Schilperoort R.A.;
RT   "Transposition of Tn904 encoding streptomycin resisatnce into the
RT   octopine Ti plasmid of Agrobacterium tumefaciens";
RL   J. Bacteriol. 141:129-136(1980).
RN   [10]
RC   pTiA6 NC from Agrobacterium tumefaciens
RA   Currier T.C., Nester E.W.;
RT   "Evidence for diverse types of large plasmids in tumor inducing
RT   strains of Agrobacterium";
RL   J. Bacteriol. 126:157-165(1976).
RN   [11]
RC   pTiB6 806 from Agrobacterium tumefaciens
RA   Drummond M.H., Chilton M.D.;
RT   "Tumor inducing (Ti) plasmids of Agrobacterium share extensive
RT   regions of homology";
RL   J. Bacteriol. 136:1178-1183(1978).
RN   [12]
RC   pGA164 from SV40 early promoter & lacZ gene
RA   Lee J.S., An G., Friesen J.D., Fiil N.P.;
RT   "Location of the tufB promoter of E. coli: cotranscription of tufB
RT   with four transfer RNA genes";
RL   Cell 25:251-261(1981).
CC   Plasmid vector for transfer of exogenous DNA into plant chromosomes.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pGA437)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli MC1061)(higher plants)(Agrobacterium tumefaciens)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pGA435 from pTiT37 & pGA430, from pGA342 & pT37H23)
CC   PA (pTJS75 from RK2)
CC   BR (pGA446)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. RK2
FT                   2. pBR322, tet gene
FT                   -> pTJS75
FT                   1. pTiT37
FT                   2. pBR322
FT                   -> pT37H23
FT                   1. Tn5 BglII-BamHI 1541bp 1516..3057, kan/neo gene
FT                   2. HSV, tk gene promoter/polyA/#X14112
FT                   -> pAG60
FT                   1. pAG60 remove EcoRI-EcoRI, HSV1 tk promoter/#X14112
FT                   -> pGA342
FT                   1. pGA342 BglII, neo/kan gene
FT                   2. pT37H23 Sau3A-Sau3A 345bp, nos promoter/gene
FT                   -> pGA355 [unique BglII between nos and neo]
FT                   1. pGA355 BglII
FT                   BAL31 nuclease
FT                   BamHI linker 10mer nnggatccnn
FT                   -> pGA355-147 [no 5' neo]
FT                   1. pT37H23 EcoRV, 5' to nos gene
FT                   SalI linker 6bp gtcgac
FT                   remove SalI-SalI, nos gene/pBR322
FT                   -> pGA428
FT                   1. pGA355-147 SacII-SmaI
FT                   2. pGA428 remove small SacII-BamHI
FT                   -> pGA430 7000bp
FT                   1. pTiT37 PstI-PstI 4000bp, left border
FT                   2. pGA430 PstI, amp gene
FT                   -> pGA435 11000bp
FT                   1. pGA435 SalI, complete
FT                   2. pTJS75 SalI, complete
FT                   -> pGA437 18300bp"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI-KpnI-BglII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;