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ID   PGAL8      preliminary; circular DNA; SYN; 8700 BP.
AC   IG5098;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pGal8 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pGal8 from pUC18 & HSV-1 tk gene
RC   pgCL3 from pGal8 & gC promoter
RC   pgEP2b from pGal8 & gE promoter
RC   pgHL.1 from pGal8 & gH promoter
RC   vgCL3 from pgCL3 & HSV-1
RC   vgEP2b from pgEP2b & HSV-1
RC   vgHL.1 from pgHL.1 & HSV-1
RA   Weir J.P., Steffy K.R., Sethna M.;
RT   "An insertion vector for the analysis of gene expression during
RT   herpes simplex virus infection";
RL   Gene 89:271-274(1990).
CC   MCS. not original sequence.
CC   NM (pGal8)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (HSV-1)(pUC18)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC18 remove XbaI-BamHI 6bp 424..430,
FT                   \ MCS/2680bp
FT                   2. E. coli XbaI-BamHI 3000bp, lacZ gene
FT                   -> plasmid 5700bp
FT                   1. plasmid remove SacI-EcoRI 2bp, MCS/pUC18 449..451
FT                   \ 5700bp
FT                   2. HSV-1 SacI-EcoRI 1800bp, 3' tk gene/#X14112
FT                   -> plasmid2 4500bp
FT                   1. plasmid2 remove SphI-BamHI 20bp, MCS/pUC18 410..430
FT                   \ 5700bp
FT                   2. HSV-1 BamHI-SphI 1300bp, 5' tk gene/#X14112
FT                   -> plasmid3 7000bp
FT                   1. plasmid3 remove small HindIII-XbaI
FT                   2. pUC18 HindIII-XbaI 24bp 400..424, MCS
FT                   -> pGal8 8700bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 140 BP; 40 A; 32 C; 31 G; 37 T; 0 other;
     acaatgggcc aagcttgcat gcctgcaggt cgactctaga gtttatttgc atacattcaa
     tcaattgtta tctaaggaag tacttacata tggttcgtgc aaacaaacgc aacgaggctc
     tacgaatcgg ggatcccgtc