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ID   PGCATA     preliminary; circular DNA; SYN; 4556 BP.
AC   ATCC37640;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pGCAT-A - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pGCAT-A, pGCAT-C from pGEM-4 & pSB1
RC   pSVGCAT-A from pGCAT-A & SV40 promoter
RC   pSVGCAT-C from pGCAT-C & SV40 promoter
RA   Frebourg T., Brison O.;
RT   "Plasmid vectors with multiple cloning sites and cat-reporter gene
RT   for promoter cloning and analysis in animal cells";
RL   Gene 65:315-318(1988).
RN   [2]
RC   pALB-cat, pSVE-cat from pSB1 & linker & rat genome JB
RC   from pSVE-cat
RC   from pRSVcat
RA   Ott M.O., Sperling L., Herbomel P., Yaniv M., Weiss M.C.;
RT   "Tissue-specific expression is conferred by a sequence from the
RT   5' end of thr rat albumin gene";
RL   EMBO J. 3:2505-2510(1984).
RN   [3]
RC   pBB6 from gpt gene & tk gene
RC   pSB1 from pBB6 & pSV2cat, cat gene
RA   Bourachot B., Jouanneau J., Giri I., Katinka M., Cereghini S.,
RA   Yaniv M.;
RT   "Both early and late control sequences of SV40 and polyoma promote
RT   transcription of Escherichia coli gpt gene in transfected cells";
RL   EMBO J. 1:895-900(1982).
RN   [4]
RC   pSVE-cat from pSB1 & SV40 early region
RA   Herbomel P., De Combrugghe B., Yaniv M.;
RT   ;
RL   Cold Spring Harb. Conf. Cell Prolif. 10:285-294(1983).
CC   Restriction digests of the clone give the following sizes (kb):
CC   PvuII--4.3, 0.2; HincII--3.0, 1.5; EcoRI--4.5. (ATCC staff)
CC   The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and
CC   pGCAT-C (ATCC 37641).
CC   The sequence at the junction of the multiple cloning site and CAT is
CC   GAATTAGCTT and at the junction of SV40 and SP6 is GGATCAATTC.
CC   (personal communication)
CC   The BamHI/HindIII fragment of pSB1 containing CAT was cloned into the
CC   EcoRI site of pGEM-4 (all ends blunt). Contains the SV40
CC   polyadenylation site and donor and acceptor sites of SV40 small t
CC   antigen intron.
CC   A plasmid shuttle vector for investigating promoter and/or enhancer
CC   elements. (ATCC staff)
CC   Has a polylinker upstream of the reporter CAT gene and demonstrates
CC   low basal CAT expression in eukaryotic cells. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. gpt gene
FT                   2. tk gene
FT                   -> pBB6
FT                   1. pSV2-cat, cat gene
FT                   2. pBB6
FT                   -> pSB1
FT                   1. pGEM-4 EcoRI 2871bp 11..11
FT                   Klenow
FT                   2. pSB1 BamHI-HindIII, cat gene
FT                   Klenow:Klenow
FT                   -> pGCAT-A 4556bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-AccI-HincII-XbaI-
FT                   BamHI-AvaI-SmaI-KpnI-SacI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SphI-PstI-SalI-AccI-XbaI-
FT                   BamHI-AvaI-SmaI-KpnI-SacI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;