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ID   PGCATC     preliminary; circular DNA; SYN; 4552 BP.
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AC   ATCC37641;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pGCAT-C - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGCAT-A, pGCAT-C from pGEM-4 & pSB1
RC   pSVGCAT-A from pGCAT-A & SV40 promoter
RC   pSVGCAT-C from pGCAT-C & SV40 promoter
RA   Frebourg T., Brison O.;
RT   "Plasmid vectors with multiple cloning sites and cat-reporter gene
RT   for promoter cloning and analysis in animal cells";
RL   Gene 65:315-318(1988).
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CC   Restriction digests of the clone give the following sizes (kb):
CC   PvuII--3.0, 1.5; HincII--3.0, 1.5; EcoRI--4.25, 0.3. (ATCC staff)
CC   The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and
CC   pGCAT-C (ATCC 37641).
CC   The sequence at the junction of the multiple cloning site and CAT is
CC   GAATTCGAGC and at the junction of SV40 and SP6 is GGATCAGCTT.
CC   (personal communication)
CC   BamHI/HindIII fragment of pSB1 (CAT) was cloned into the HindIII site
CC   of pGEM-4. 2 hrs after ligation began the remaining BamHI and HindIII
CC   sites were filled in. Contains SV40 polyadenylation site and donor and
CC   acceptor sites of SV40 small-t intron.
CC   A plasmid shuttle vector for investigating promoter and/or enhancer
CC   elements. Has a polylinker upstream of reporter CAT gene, SP6 and T7
CC   promoters for in vitro RNA synthesis and low basal CAT expression in
CC   eukaryotic cells.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pGCAT-C)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. gpt gene
FT                   2. tk gene
FT                   -> pBB6
FT                   1. pSV2-cat, cat gene
FT                   2. pBB6
FT                   -> pSB1
FT                   1. pGEM-4 HindIII 2871bp 62..62
FT                   Klenow:
FT                   2. pSB1 BamHI-HindIII, cat gene
FT                   Klenow:
FT                   -> pGCAT-A 4552bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-AvaI-BamHI-XbaI-
FT                   HincII-AccI-SalI-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-KpnI-SmaI-AvaI-BamHI-XbaI-
FT                   AccI-SalI-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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