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ID   PGHQ1      preliminary; circular DNA; SYN; 212 BP.
AC   M13705;
DT   16-FEB-1987 (Rel. 2, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pGHQ1 and pGHR1 - incomplete, let/interferon.
KW   cloning vector; leftward lambda promoter; E.coli trp promoter;
KW   let promoter; interferon; interferon-gamma.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-212
RC   pPLT4 from pKYP100 & pMY12-6
RC   pPLA201 from pBR322 & pKYP100 & pHUB2
RC   pHUB2 from lambda, pL promoter; [from Enzobiochem Inc]
RC   pGBK3 from pGKA2 & pKYP14
RC   pGDD4 from pGBK3 & pKYP100
RC   pGDY1 from pGBK3 & pKYP401
RC   pGHA2 from pGKA2 & pPLT4
RC   pGHQ1 from pGHA2 & pGBK3
RC   pGHJ1 from pGHA2 & pPLA201, pMY12-6
RC   pGHR1 from pGHJ1 & pGBK3
RC   pCR3 from pMY12-6 & pBR328
RC   pGHN3 from pGHA2 & pCR3
RA   Nishi T., Itoh S.;
RT   "Enhancement of transcriptional activity of the Escherichia coli trp
RT   promoter by upstream A + T-rich regions";
RL   Gene 44:29-36(1986).
RN   [2]
RP   1-212
RC   pGHQ1
RA   Nishi T.;
RT   ;
RL   Submitted (18-NOV-1986) by:
RL   Nishi T., .
RN   [3]
RC   lambda cI857 trpED10 from lambda & E.coli trp promoter
RC   pKYP1 from pBR325 & lambda cI857 trpED10, E.coli trp promoter
RC   pKYP5 from pBR322 & pKYP1
RC   pKYP10, pKYP11 from pBR322 & pKYP5
RC   pKYP100 from pKYP10 & linker & lambda cI857 trpED10, E.coli trp prom
RC   pKYP101 from pKYP10 & linker & lambda cI857 trpED10, E.coli trp prom
RC   pKYP20 from pKYP10 & linker & lambda cI857 trpED10, E.coli trp prom
RC   pKYP400 from pKYP100 & pBR328
RC   pKYP401 from pKYP400 & pKYP100
RC   pKYP15, pKYP21 from pKYP10
RC   pKYP11 from pKYP10 & lambda & E.coli trp promoter
RC   pKYP12 from pKYP11 & lambda & E.coli trp promoter
RC   pKYP200 from pKYP100
RC   pKYP14 from pKYP10 & pIN-II-A2
RC   pGBK3 from pKYP14 & pGKA2, human gamma interferon
RA   Nishi T., Saito A., Oka T., Itoh S., Takaoka C., Taniguchi T.;
RT   "Construction of plasmid expression vectors carrying the
RT   Escherichia coli tryptophan promoter";
RL   Agric. Biol. Chem. 48:669-675(1984).
RN   [4]
RC   pIFNgamma-5A, pIFNgamma-G4 from p3-2-1 & human gamma interferon
RC   pHGgammaG4A, pHGgammaG4A from pKCR & pIFNgamma-G4
RC   pGC7 from pHGgammaG4A & pKYP11
RC   pGKA2 from pGC7 & pKYP10
RC   pGAZ2 from pGKA2 & pIFNgamma-5A
RA   Nishi T., Fujita T., Nishi-Takaoka C., Saito A., Matsumoto T.,
RA   Sato M., Oka T., Itoh S., Yip Y.K., Vilcek J., Taniguchi T.;
RT   "Cloning and expression of a novel variant of human
RT   interferon-gamma cDNA";
RL   J. Biochem. 97:153-159(1985).
RN   [5]
RC   p3-2-1 from pBR322 & SV40 & linker
RA   Oda K., Kato H., Saito I., Sugano S., Maruyama K., Masuda M.,
RA   Shiroki K., Shimojo H.;
RT   "Expression of adenovirus type 12 E1A gene in monkey cells, using
RT   a simian virus 40 vector";
RL   J. Virol. 45:408-419(1983).
CC   The deletion of the two 'at'-rich blocks from the E.coli trp
CC   promoter resulted in decreased promoter strength.  By replacing
CC   these 'at'-rich blocks with one or two large 'at'-rich blocks from
CC   the major leftward lambda promoter (p-L), modified trp promoters
CC   (letI, with one, or letII with two 'at'-rich blocks) were found to
CC   be several times more effective than the wild-type trp promoter.
CC   The letI promoter can be repressed by the trp repressor and the
CC   lambda repressor.  A -35-like sequence is located at positions
CC   160-172.
CC   NCBI gi: 208769
CC   NM (pGHQ1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pKYP100)(p-P-L-T4)
CC   BR (pGHR1)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII-PvuII, ori
FT                   2. SV40 HindIII-PvuII 0.945 to 0.710
FT                   remove EcoRI-AvaII 0.928 to 0.771
FT                   HindIII linker
FT                   -> p3-2-1
FT                   1. p3-2-1 KpnI
FT                   poly(dG-dC) tailing
FT                   2. human gamma interferon 1000bp
FT                   poly(dG-dC) tailing
FT                   -> pIFNgamma-G4 [RsaI near BstNI in interferon]
FT                   1. pKCR BamHI, 5' to SV40
FT                   2. pIFNgamma-G4 Sau3AI-Sau3AI, interferon
FT                   -> pHGgammaG4A
FT                   1. lambda
FT                   2. E.coli trp promoter
FT                   -> lambda cI857 trpED10
FT                   1. pBR325
FT                   2. lambda cI857 trpED10, E.coli trp promoter
FT                   -> pKYP1
FT                   1. pBR322
FT                   2. pKYP1, human gamma interferon gene/trpAT2 promoter
FT                   -> pKYP5
FT                   1. pBR322
FT                   2. pKYP5, human gamma interferon gene/trpAT2 promoter
FT                   -> pKYP10
FT                   1. pBR322
FT                   2. pKYP5, human gamma interferon gene/trpAT2 promoter
FT                   -> pKYP11
FT                   1. pKYP11 BamHI-HindIII
FT                   Klenow fill in:
FT                   2. pHGgammaG4A HindIII-PvuII
FT                   -> pGC7
FT                   1. pGC7 BstNI-SalI
FT                   2. pKYP10 remove HindIII-SalI
FT                   -> pGKA2
FT                   1. pKYP10
FT                   2. linker
FT                   3. lambda cI857 trpED10, E.coli trp promoter
FT                   -> pKYP100 [PstI-HindIII 880bp, trpAT2 promoter]
FT                   1. pKYP100 XhoI
FT                   fill in
FT                   2. pMY12-6 BglII-BamHI 250bp, pL lambda 35711..35466
FT                   fill in:fill in
FT                   -> pPLT4 [unique EcoRI]
FT                   1. pGKA2 PstI-HincII 4200bp, trp promoter
FT                   2. pPLT4 PstI-HincII 860bp, 5' pL promoter
FT                   -> pGHA2
FT                   1. pGKA2 remove small BamHI-SalI
FT                   2. pKYP14 BamHI-SalI 950bp, lpp terminator
FT                   -> pGBK3
FT                   1. pGHA2 PstI-HindIII 920bp, letI promoter
FT                   2. pGBK3 remove PstI-HindIII
FT                   -> pGHQ1"
FT   promoter        1..212
FT                   /note="PRO E. coli letII gene"
FT   misc_feature    12..56
FT                   /note="AT-rich block A"
FT   misc_feature    78..212
FT                   /note="PRO E. coli letI gene"
FT   misc_feature    110..136
FT                   /note="AT-rich block B"
FT   -35_signal      174..185
FT                   /note="S35 E. coli trpO gene"
FT   misc_recomb     177..178
FT                   /note="bacteriophage lambda DNA end/E.coli DNA start"
FT   misc_feature    191..210
FT                   /note="PRO E. coli trpO operator"
FT   -10_signal      199..205
FT                   /note="S10 E. coli trpO gene"
FT   misc_feature    212..>212
FT                   /note="human interferon-gamma, untranslatable EMBL CDS
FT                   feature"
SQ   Sequence 212 BP; 86 A; 50 C; 24 G; 52 T; 0 other;
     cgatcgcccc caaaacacat aaccaattgt atttattgaa aaataaatag atacaactca
     ctaaacatag caattcagat ctctcaccta ccaaacaatg cccccctgca aaaaataaat
     tcatataaaa aacatacaga taaccatctg cggtgataaa ttatctctgg cggtgttgac
     aattaatcat cgaactagtt aactagtacg ca