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ID   PGINT102   preliminary; circular DNA; SYN; 1 BP.
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AC   IG5115;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pGint102 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pG15 from pBR322 & pGE1, VSV G protein gene
RC   pGint20 from pG15 & pJC119
RC   pGint61 from pGint20 & pBR322
RC   pGint102 from pGint20 & pBR322
RA   Rottier P.J., Florkiewicz R.Z., Shaw A.S., Rose J.K.;
RT   "An internalized amino-terminal signal sequence retains full
RT   activity in vivo but not in vitro";
RL   J. Biol. Chem. 262:8889-8895(1987).
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RN   [2]
RC   pAS621 from pBR322 & linker & trpE gene
RC   pAS621 from pAS620
RC   plasmid from pBR325 & linker & pG1, VSV G protein gene
RC   pGE1, pGE2 from pAS621 & plasmid
RC   pG1-modified from pG1
RC   pGE4 from pAS621 & pG1-modified
RA   Rose J.K., Shafferman A.;
RT   "Conditional expression of the vesicular stomatitis virus
RT   glycoprotein gene in Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:6670-6674(1981).
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RN   [3]
RC   pG1 from pBR322 & VSV G protein gene
RA   Rose J.K., Gallione C.;
RT   "Nucleotide sequences of the mRNA's encoding the vesicular
RT   stomatitis virus G and M proteins determined from cDNA clones
RT   containing the complete coding regions";
RL   J. Virol. 39:519-528(1981).
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CC   not original seqeunce. signal sequence.
CC   NM (pGint102)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pG15)(pBR322)(pGE1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 PstI
FT                   dC tailing
FT                   2. VSV PstI-PstI, G protein gene
FT                   dG tailing
FT                   -> pG1
FT                   1. pBR325 EcoRI
FT                   2. pG1 PstI-PstI, VSV G protein gene
FT                   BAL31 nuclease, remove 50bp at each end
FT                   EcoRI linker:EcoRI linker
FT                   EcoRI-EcoRI
FT                   -> plasmid
FT                   1. pBR322 EcoRI
FT                   2. E. coli HinfI-HinfI 497bp, trp pro/op/lead/att/trpE
FT                   EcoRI linker:EcoRI linker
FT                   EcoRI-EcoRI
FT                   -> pAS620
FT                   1. pAS620 EcoRI, 5' to trp
FT                   Klenow fill in
FT                   -> pAS621
FT                   1. pAS621 EcoRI, 3' to trp
FT                   2. plasmid EcoRI-EcoRI, VSV G protein gene
FT                   -> pGE1
FT                   1. pBR322 EcoRI
FT                   2. pGE1 EcoRI-EcoRI, VSV G protein gene
FT                   BAL31 nuclease
FT                   -> pG15
FT                   1. pG15 ThaI-EcoRI, VSV G protein gene
FT                   :Klenow fill in
FT                   XhoI linker:XhoI linker
FT                   XhoI-XhoI
FT                   2. pJC119 XhoI
FT                   -> pGint20
FT                   1. pGint20 XmnI
FT                   2. pBR322 small HaeIII-HaeIII
FT                   -> pGint102"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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