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ID   PGIP21     preliminary; circular DNA; SYN; 5200 BP.
AC   IG5075;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pGIP21 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pGIP21 from pUC18 & pRP1, B.licheniformis amyL gene
RC   pGIP61 from pGIP21 & pVA838
RC   [pGIP63 from pGIP21]
RC   plasmid from pGIP21 & pMTL23P
RC   plasmid2 from plasmid & pGIP63
RC   pGIP312 from plasmid2 & pNZ12, broad host range
RC   pGIP212 from plasmid2 & pGK13, broad host range
RA   Hols P., Baulard A., Garmyn D., Delplace B., Hogan S., Delcour J.;
RT   "Isolation and characterization of genetic expression and secretion
RT   signals from Enterococcus faecalis through the use of
RT   broad-host-range alpha-amylase probe vectors";
RL   Gene 118:21-30(1992).
RN   [2]
RC   pRP1 from pBD64 & B.licheniformis amyL gene
RA   Piggott R.P., Rossiter A., Ortlepp S.A., Pembroke J.T.,
RA   Ollington J.F.;
RT   "Cloning in Bacillus subtilis of an extremely thermostable alpha
RT   amylase: comparison with other cloned heat-stable alpha amylases";
RL   Biochem. Biophys. Res. Commun. 122:175-183(1984).
RN   [3]
RC   pNZ12 from Lactococcus ori
RA   De Vos W.M.;
RT   "Gene cloning in lactic streptococci";
RL   Neth. Milk Dairy J. 40:141-154(1986).
RN   [4]
RC   pSA33 from amyL gene
RA   Ortlepp S.A., Ollington J.F., McConnell D.J.;
RT   "Molecular cloning in Bacillus subtilis of a BAcillus licheniformis
RT   gene encoding a thermostable alpha amylase";
RL   Gene 23:267-276(1983).
RN   [5]
RC   pAMY2 from amyL gene
RA   Cornelis P., Digneffe C., Willemot K.;
RT   "Cloning and expression of a Bacillus coagulans amylase gene in
RT   Escherichia coli";
RL   Mol. Gen. Genet. 186:507-511(1982).
CC   not original sequence.
CC   NM (pGIP21)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pRP1)(pUC18)
CC   BR (pGIP61)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC18 BamHI, MCS
FT                   2. pRP1 BclI-BclI 2500bp, B.licheniformis amyL gene
FT                   -> pGIP21 5200bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;