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ID   PGIP2121   preliminary; circular DNA; SYN; 302 BP.
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AC   M77275; M77276;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pGIP2121 - incomplete, fusion.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-302 M77275; 1-372 M77276
RC   pGIP21 from pUC18 & pRP1, B.licheniformis amyL gene
RC   pGIP61 from pGIP21 & pVA838
RC   [pGIP63 from pGIP21]
RC   plasmid from pGIP21 & pMTL23P
RC   plasmid2 from plasmid & pGIP63
RC   pGIP312 from plasmid2 & pNZ12, broad host range
RC   pGIP212 from plasmid2 & pGK13, broad host range
RA   Hols P., Baulard A., Garmyn D., Delplace B., Hogan S., Delcour J.;
RT   "Isolation and characterization of genetic expression and secretion
RT   signals from Enterococcus faecalis through the use of
RT   broad-host-range alpha-amylase probe vectors";
RL   Gene 118:21-30(1992).
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CC   NCBI gi: 488329
CC   NM (pGIP2121)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pRP1)(pUC18)
CC   BR (pGIP61)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHP45omega remove small BamHI-BamHI
FT                   fill in: fill in
FT                   2. phage T4 BamHI-BamHI, gene 32 terminator
FT                   -> pGIP63 4200bp
FT                   1. pGIP21 PstI-SmaI, amyL gene
FT                   2. pMTL23P remove PstI-SmaI, MCS
FT                   -> plasmid
FT                   1. plasmid NaeI, 5' to amyL gene
FT                   2. pGIP63 SmaI-SmaI, phage T4 gene 32 terminator
FT                   -> plasmid2
FT                   1. plasmid2 PvuII-BglII, amyL gene
FT                   2. pGK13 remove small EcoRV-BamHI
FT                   -> pGIP212
FT                   1. pGIP212 BamHI-BamHI, amyL gene
FT                   2. E. faecalis DNA MboI-MboI, ori
FT                   -> pGIP2121"
FT   -35_signal      12..17
FT                   /note="S35"
FT   -10_signal      34..39
FT                   /note="S10"
FT   RBS             92..96
FT                   /note="RBS"
FT   sig_peptide     105..296
FT                   /note="other 3' end of signal peptide could be at 290"
FT   mat_peptide     300..>302
FT                   /note="E. coli alpha-amylase fusion protein"
FT   CDS             105..>302
FT                   /note="GEN E. coli alpha-amylase fusion protein;
FT                   NCBI gi: 488330"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 302 BP; 98 A; 34 C; 75 G; 95 T; 0 other;
     ttttataaaa gttgaaagtt ttataaattt tactaaaatc tgtaattttc atgaaaatat
     agcgtatgat aatgctgtta atagaatagg cggaggtaag cggtatgcgt aaacgacatg
     caaagaaaag acatggagga gtgaattggc tttttatagt atgtttgttg gtggtgattg
     gtggtagtgg ttatttaata aaaacgttct ttttcactag agattcacaa gttagtcaag
     aatcgaaagt ggtcttggaa gaagatccat atgacgtcga cgcgtctgca gcagcggcgg
     ca
//