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ID   PGIP2124   preliminary; circular DNA; SYN; 739 BP.
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AC   M77277;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pGIP2124 - incomplete, fusion.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-739
RC   pGIP21 from pUC18 & pRP1, B.licheniformis amyL gene
RC   pGIP61 from pGIP21 & pVA838
RC   [pGIP63 from pGIP21]
RC   plasmid from pGIP21 & pMTL23P
RC   plasmid2 from plasmid & pGIP63
RC   pGIP312 from plasmid2 & pNZ12, broad host range
RC   pGIP212 from plasmid2 & pGK13, broad host range
RA   Hols P., Baulard A., Garmyn D., Delplace B., Hogan S., Delcour J.;
RT   "Isolation and characterization of genetic expression and secretion
RT   signals from Enterococcus faecalis through the use of
RT   broad-host-range alpha-amylase probe vectors";
RL   Gene 118:21-30(1992).
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CC   NCBI gi: 488333
CC   NM (pGIP2124)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHP45omega remove small BamHI-BamHI
FT                   fill in: fill in
FT                   2. phage T4 BamHI-BamHI, gene 32 terminator
FT                   -> pGIP63 4200bp
FT                   1. pGIP21 PstI-SmaI, amyL gene
FT                   2. pMTL23P remove PstI-SmaI, MCS
FT                   -> plasmid
FT                   1. plasmid NaeI, 5' to amyL gene
FT                   2. pGIP63 SmaI-SmaI, phage T4 gene 32 terminator
FT                   -> plasmid2
FT                   1. plasmid2 PvuII-BglII, amyL gene
FT                   2. pGK13 remove small EcoRV-BamHI
FT                   -> pGIP212
FT                   1. pGIP212 BamHI-BamHI, amyL gene
FT                   2. E. faecalis DNA MboI-MboI, ori
FT                   -> pGIP2123
FT                   1. pGIP2123 remove HindIII-ClaI 700bp, 5' amyL gene
FT                   -> pGIP2124"
FT   -35_signal      326..330
FT                   /note="S35"
FT   -10_signal      354..359
FT                   /note="S10"
FT   RBS             385..394
FT                   /note="RBS"
FT   sig_peptide     401..484
FT                   /note="other 3' end of signal peptide could be at 290"
FT   mat_peptide     737..>739
FT                   /note="E. coli alpha-amylase fusion protein"
FT   CDS             401..>739
FT                   /note="GEN E. coli alpha-amylase fusion protein;
FT                   NCBI gi: 488330"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 739 BP; 302 A; 109 C; 140 G; 188 T; 0 other;
     atcgataccg tcgacggtat cgattaaatt tccaacaacg tcgttttatt aacaattacc
     caaaactaac tttaaaaaac attaaaaatt ccaaagaagt gcaacatctc ttagaagatt
     tgaagaaaaa gggccaataa ttaacaaaaa atattacaaa atatttcaat caatggtcta
     atgggatgaa aaaaagaaaa atcgtcaaaa gctacttttt aaggcgtctt aagaaattct
     taaagaattg agatagaaat ggactaatga ataaacgcag gataaaaaaa gagttgcatt
     taaattgtaa caaattgtaa catgattgaa ataaaagaag ccctcctgac tagtataatg
     agtaggtgtt ttagataagt taggaaaggt gttccattta gtgaaggtta aaaagttgat
     accattttta acaacaatga tgcttatcaa ttttggactt ccagtcgctg cggcagcaga
     atcacttgat tcgttgcaag aaaaagaagg ccaagcgaaa caatctgggg agttaattag
     taaagaaatc aattcagcct tagacgatgt aaatgctaaa tatgcggaaa tcgaacgttt
     aaaaacgcaa atttcaaaag cggaagatac gttgaaaact tcgaaagagg aaatagaaaa
     aacagaaaaa aatatttctc gtcgtaaaga agctgttggc gatccatatg acgtcgacgc
     gtctgcagca gcggcggca
//