back Return to this vector's summary.
ID   PGIP2563   preliminary; circular DNA; SYN; 474 BP.
XX
AC   M77278;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGIP2563 - incomplete, fusion.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-474
RC   pGIP21 from pUC18 & pRP1, B.licheniformis amyL gene
RC   pGIP61 from pGIP21 & pVA838
RC   [pGIP63 from pGIP21]
RC   plasmid from pGIP21 & pMTL23P
RC   plasmid2 from plasmid & pGIP63
RC   pGIP312 from plasmid2 & pNZ12, broad host range
RC   pGIP212 from plasmid2 & pGK13, broad host range
RA   Hols P., Baulard A., Garmyn D., Delplace B., Hogan S., Delcour J.;
RT   "Isolation and characterization of genetic expression and secretion
RT   signals from Enterococcus faecalis through the use of
RT   broad-host-range alpha-amylase probe vectors";
RL   Gene 118:21-30(1992).
XX
CC   NCBI gi: 488333
CC   NM (pGIP2563)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGIP3124 HindIII-RsaI, E.faecalis promoter
FT                   2. oligo ggaggatg
FT                   -> plasmid
FT                   1. plasmid
FT                   2. pGK13
FT                   -> pGIP2563"
FT   -35_signal      48..53
FT                   /note="S35"
FT   -10_signal      74..79
FT                   /note="S10"
FT   RBS             164..170
FT                   /note="RBS"
FT   mat_peptide     472..>474
FT                   /note="ORFB"
FT   CDS             178..363
FT                   /note="ORF"
FT   CDS             433..>474
FT                   /note="ORFB"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 474 BP; 161 A; 74 C; 94 G; 145 T; 0 other;
     agcttgtttc ttttacttta aaattccatt acaaaatgag gatttttttg aaaaatcaat
     cggctcagtt gttttttatt aaattaaggt ccggccagct tacctattcc ccaaagaaaa
     taaataggtt gttcaaataa attgtggtaa acttgataca ttaaaaggag ggagaatatg
     cgcccaaaag agaaaaaaag aggaaaaaat tggttaatca acagtttatt agttttacta
     tttatcattg gcttggcctt aatttttaac aatcagatac gcagttgggt ggttcaacaa
     aatagccgct cgtatcgaat tcgagctcgg tacccggcgc ttttcttttg gaagaaaata
     tagggaaaat ggtacttgtt aaaaattcgg aatatttata caacatcata atcggatctg
     gaggaaaaaa ttatggatcc atatgacgtc gacgcgtctg cagcagcggc ggca
//