ID   PGIP3124   preliminary; circular DNA; SYN; 588 BP.
XX
AC   M77279;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pGIP3124 - incomplete, fusion.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-588
RC   pGIP21 from pUC18 & pRP1, B.licheniformis amyL gene
RC   pGIP61 from pGIP21 & pVA838
RC   [pGIP63 from pGIP21]
RC   plasmid from pGIP21 & pMTL23P
RC   plasmid2 from plasmid & pGIP63
RC   pGIP312 from plasmid2 & pNZ12, broad host range
RC   pGIP212 from plasmid2 & pGK13, broad host range
RA   Hols P., Baulard A., Garmyn D., Delplace B., Hogan S., Delcour J.;
RT   "Isolation and characterization of genetic expression and secretion
RT   signals from Enterococcus faecalis through the use of
RT   broad-host-range alpha-amylase probe vectors";
RL   Gene 118:21-30(1992).
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CC   NCBI gi: 488333
CC   NM (pGIP3124)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGIP312 remove small BamHI-BamHI 1700bp
FT                   2. E. faecalis DNA Sau3A-Sau3A, ori
FT                   -> pGIP3121
FT                   1. pGIP3121 HindIII-HindIII 1100bp, 5' amyL gene
FT                   -> pGIP3124"
FT   -35_signal      48..53
FT                   /note="S35"
FT   -10_signal      74..79
FT                   /note="S10"
FT   RBS             164..171
FT                   /note="RBS"
FT   sig_peptide     178..324
FT                   /note="other 3' ends of signal peptide could be at
FT                   285, 312 and 318"
FT   mat_peptide     586..>588
FT                   /note="E. coli alpha-amylase fusion protein"
FT   CDS             178..>588
FT                   /note="E. coli alpha-amylase fusion protein"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 588 BP; 194 A; 100 C; 122 G; 172 T; 0 other;
     agcttgtttc ttttacttta aaattccatt acaaaatgag gatttttttg aaaaatcaat
     cggctcagtt gttttttatt aaattaaggt ccggccagct tacctattcc ccaaagaaaa
     taaataggtt gttcaaataa attgtggtaa acttgataca ttaaaaggag ggagaatatg
     cgcccaaaag agaaaaaaag aggaaaaaat tggttaatca acagtttatt agttttacta
     tttatcattg gcttggcctt aatttttaac aatcagatac gcagttgggt ggttcaacaa
     aatagccgct cgtacgccgt tagcaagttg aaaccagccg atgtgaagaa aaatatggct
     cgtgaaacaa cgtttgactt tgattcagtt gagtccttga gcacagaagc ggtgatgaaa
     gcccaatttg aaaacaaaaa cttacctgtg attggtgcca ttgcgatacc aagtgtcgaa
     attaatttgc ccatttttaa aggattgtca aatgtcgctt tattaactgg tgccgggacc
     atgaaagaag atccatatga cgtcgacgcg tctgcagcag cggcggca
//