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ID   PGK1       preliminary; circular DNA; SYN; 2400000 BP.
XX
AC   IG7011;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   Streptococcus/Bacillus/E.coli plasmid vector pGK1 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGK1 from pWV01 & pC194
RC   pGK12 from pGK1 & pE194
RC   pGK13 from pGK1 & pE194
RC   pGK11 from pGK1 & pE194
RC   [pBK11 from pGK1]
RA   Kok J., Van Der Vossen J.M.B.M., Venema G.;
RT   "Construction of plasmid cloning vectors for lactic Streptococci
RT   which also replicate in Bacillus subtilis and Escherichia coli";
RL   Appl. Environ. Microbiol. 48:726-731(1984).
XX
CC   NM (pGK1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Bacillus)(Streptococcus)
CC   CP ()
CC   FN (shuttle)
CC   SE ()
CC   PA (pWV01)(pC194)
CC   BR ()
CC   OF (pGK12)
CC   OR (yes)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pWV01
FT                   2. pC194, cat gene
FT                   -> pGK1 24000000bp"
FT   rep_origin      complement(0..0)
FT                   /note="ORI Streptococcus cremoris Wg2
FT                   (1500000 bp cryptic plasmid)"
FT   CDS             0..0
FT                   /note="ANT Bacillus chloramphenicol resistance gene
FT                   (cat), from pC194"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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