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ID   PGK12      preliminary; circular DNA; SYN; 2900000 BP.
AC   IG7012;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Streptococcus/Bacillus/E.coli plasmid vector pGK12 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pGK1 from pWV01 & pC194
RC   pGK12 from pGK1 & pE194
RC   pGK13 from pGK1 & pE194
RC   pGK11 from pGK1 & pE194
RC   [pBK11 from pGK1]
RA   Kok J., Van Der Vossen J.M.B.M., Venema G.;
RT   "Construction of plasmid cloning vectors for lactic Streptococci
RT   which also replicate in Bacillus subtilis and Escherichia coli";
RL   Appl. Environ. Microbiol. 48:726-731(1984).
CC   NM (pGK12)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Bacillus)(Streptococcus)
CC   CP (5 in Bacillus)(60 in E.coli)(3 in Streptococcus)
CC   FN (shuttle)(insertion inactivation)
CC   SE ()
CC   PA (pGK1)(pE194 cop-6)(pGL112)
CC   BR (pGK11)
CC   OF (pGhost4)
CC   OR (yes)
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pGK1 2400000bp
FT                   2. pE194 cop-6, ery gene
FT                   -> pGK12 29000000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaII"
FT   CDS             0..0
FT                   /note="ANT Bacillus erythromycin resistance gene
FT                   (ery), from pE194 cop-6"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI Streptococcus cremoris Wg2
FT                   (1500000 bp cryptic plasmid), from pGK1"
FT   CDS             0..0
FT                   /note="ANT Bacillus chloramphenicol resistance gene
FT                   (cat), from pC194, from pGK1"
FT   misc_feature    0..0
FT                   /note="pGL112 fragment"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;