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ID   PGKV210    preliminary; circular DNA; SYN; 2900000 BP.
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AC   IG7013;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Streptococcus/Bacillus/E.coli plasmid vector pGKV210 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGK3 from pWV01 & pE194 cop-6
RC   pGKV1, pGKV2 from pGK3 & pPL608
RC   pGKV10, pGKV11 from pGKV1
RC   pGKV20, pGKV21 from pGKV2
RC   pGKV110 from pGKV10 & M13mp11
RC   pGKV210 from pGKV20 & M13mp11
RC   pGKV12, pGKV13 from pGKV11 & pPLP1
RA   Van Der Vossen J.M., Kok J., Venema G.;
RT   "Construction of cloning, promoter-screening, and
RT   terminator-screening shuttle vectors for Bacillus subtilis and
RT   Streptococcus lactilis";
RL   Appl. Environ. Microbiol. 50:540-542(1985).
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RN   [2]
RC   pPLP1 from pPL608 & B.licheniformis pen gene
RA   Van Randen J.;
RT   ;
RL   Unpublished (1985).
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CC   NM (pGKV210)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Lactococcus lactis)(Bacillus)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pWV01)(M13mp11)(pPL603)(pE194 cop-6)
CC   BR ()
CC   OF (pGKV221)(pGKV223)(pGKV232)(pGKV244)(pGKV259)(pGKV432 from pGK232)
CC   OR (yes)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pE194 cop-6 ClaI-HpaII, ery gene
FT                   2. pWV01 ClaI-ClaI, largest
FT                   -> pGK3 1900000bp
FT                   1. pPL608 PvuII-EcoRI, SPO2 prom/cat gene [3300000bp]
FT                   :Klenow, fill in
FT                   2. pGK3 ClaI-ClaI
FT                   Klenow, fill in:Klenow, fill in
FT                   -> pGKV2 3100000bp [EcoRI]
FT                   1. pGKV2 EcoRI-EcoRI, largest
FT                   -> pGKV20 2900000bp
FT                   1. pGKV20 remove small EcoRI-PstI
FT                   2. M13mp11 small EcoRI-PstI
FT                   -> pGKV210 2900000bp
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI, from M13mp11"
FT   misc_feature    0..0
FT                   /note="from pPL603"
FT   CDS             0..0
FT                   /note="GEN Bacillus chloramphenicol resistance gene
FT                   (cat-86), promoter-less, from pPL603; #K00544"
FT   misc_binding    0..0
FT                   /note="SIT BclI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_feature    0..0
FT                   /note="from pWV01"
FT   rep_origin      0..0
FT                   /note="ORI Lactococcus lactis cremoris Wg2 pWV101"
FT   misc_feature    0..0
FT                   /note="from pE194 cop-6"
FT   CDS             complement(0..0)
FT                   /note="ANT Bacillus erythromycin resistance gene
FT                   (ery), from pPE194 cop-6"
FT   misc_binding    0..0
FT                   /note="SIT BclI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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