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ID   PGKV500    preliminary; circular DNA; SYN; 7400000 BP.
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AC   IG7014;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Streptococcus/Bacillus plasmid vector pGKV500 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pWV05 from pWV01
RC   pGD6 from pBR329 & pWV05
RC   pGD4 from pACYC184 & pWV05
RC   pGKV500 from pGKV2 & pWV05
RA   Kok J., Van Dijl J.M., Van Der Vossen J.M., Venema G.;
RT   "Cloning and expression of a Streptococcus cremoris proteinase in
RT   Bacillus subtilis and Streptococcus lactilis";
RL   Appl. Environ. Microbiol. 50:94-101(1985).
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CC   NM (pGKV500)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Lactococcus lactis)(Bacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pWV05)
CC   BR ()
CC   OF (pGD4 with pACYC184)
CC   OR (yes)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGKV2 HindIII
FT                   2. pWV05 HindIII-HindIII 4300000bp
FT                   -> pGKV500 7400000bp"
FT   misc_feature    0..0
FT                   /note="from pWV05"
FT   rep_origin      0..0
FT                   /note="ORI Lactococcus lactis cremoris Wg2 pWV01"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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