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ID   PGP704     preliminary; circular DNA; SYN; 1 BP.
AC   IG5109;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pGP704 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   p18Sfi from pUC18
RC   p18Not from pUC18
RC   pUC18Sfi from pUC18
RC   pUC18Not from pUC18
RC   pUT from pGP704 & Tn5-IS50R tnp gene
RC   pBOR7 from pUC19 & linker
RC   pBOR8 from pGP704 & pMJR1560
RC   pBOR10 from pBOR7 & pDU1358
RC   pBOR11 from p18Sfi & pHG106
RC   pBOR12 from pUC18Sfi & pUM3
RC   [pLOF from pGP704]
RC   pLOF series from pLOF
RC   pUT series from pUT
RC   pABOR70 from pJOE810 & pHG106
RA   Herrero M., De Lorenzo V., Timmis K.N.;
RT   "Transposon vectors containing non-antibiotic resistance selection
RT   markers for cloning and stable chromosomal insertion of foreign
RT   genes in gram-negative bacteria";
RL   J. Bacteriol. 172:6557-6567(1990).
RN   [2]
RC   pVC4 from pMLB1034 & pABN15
RC   pCON1 from pMLB1034 & pABN15
RC   from pMLB1034 & pABN40
RC   from pMON2064 & E. coli K-12 fur gene
RC   from pABN40 & E. coli K-12 fur gene
RC   pDB16, pDB19, pDB37 from pMLB1034 & ColV-K30, aerobactin promoter
RA   De Lorenzo V., Wee S., Herrero M., Neilands J.B.;
RT   "Operator sequences of the aerobactin operon of plasmid ColV-K30
RT   binding the ferric uptake regulation (fur) repressor";
RL   J. Bacteriol. 169:2624-2630(1987).
RN   [3]
RC   pMJR1550 from pUC18 & pMC7, lacIq gene
RC   pMJR1560 from pMJR1550 & pMC7, lacIq gene
RC   pMJR1700 from pMJR1550 & pMC7, lacIq gene
RC   pMJR1750 from pMJR1700 & oligo
RC   pUCtac from pUC18 & ptac12
RC   pUCtacT from pUCtac & pEA302T, rrnB terminator
RC   pUC8Cla from pUC8
RC   pUC9Cla from pUC9
RC   pUC18Cla from pUC18
RC   pUC19Cla from pUC19
RC   pTT8 from pUCtacT & pUC8Cla
RC   pTT9 from pUCtacT & pUC9Cla
RC   pTT18 from pUCtacT & pUC18Cla
RC   pTT19 from pUCtacT & pUC19Cla
RC   pTTQ8 from pTT8 & pMJR1560
RC   pTTQ9 from pTT9 & pMJR1560
RC   pTTQ18 from pTT18 & pMJR1560
RC   pTTQ19 from pTT19 & pMJR1560
RC   pTTQ181 from pTTQ18 & pMJR1750
RC   pMJR1600 from pTTQ18 & pUC4K
RC   pMJR1500 from pTT18 & pUC4K
RC   pMJR1610 from pUC18 & pUC4K
RC   pMJR1700 from pTTQ18 & pMLB1034
RC   pMJR1750 from pTTQ181 & pMLB1034
RC   pMJR1720 from pUC18 & pMLB1034
RC   pMJR1820 from pTTQ18 & pCM4
RA   Stark M.J.;
RT   "Multicopy expression vectors carrying the lac repressor gene for
RT   regulated high-level expression of genes in Escherichia coli";
RL   Gene 51:255-267(1987).
RN   [4]
RC   pBG195, pBG196 from pUC19 & pBG4, bar gene
RC   pBG106 from pBG195 & pIJ680
RC   pBG93, pBG94 from pUC19 & pBG195
RC   pBG20 from pBG3 & pIJ385
RA   Thompson C.J., Movva N.R., Tizard R., Grameri R., Davies J.E.,
RA   Lauwereys M., Botterman J.;
RT   "Characterization of the herbicide-resistance gene bar from
RT   Streptomyces hygroscopicus";
RL   EMBO J. 6:2519-2523(1987).
RN   [5]
RC   pDU1161 from pDU1358 & R100
RC   pDU1053 from pDU1003
RC   pHG102, pHG103 from pBR322 & pDU1358, mer genes
RC   pHG106 from pHG103 & pBR322
RC   pHG108, pHG111 from pHG102 & pHG106
RC   pHG114, pHG115 from pHG106
RC   from pUC19 & pDU1358, mer genes
RC   from pACYC184 & pDU1358, mer genes
RA   Griffin H.G., Foster T.J., Silver S., Misra T.K.;
RT   "Cloning and DNA sequence of the mercuric- and
RT   organomercurial-resistance determinants of plasmid pDU1358";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:3112-3116(1987).
RN   [6]
RC   pUM6 from pUM3
RC   pUM7 from pUM3 & pBR322
RC   pUM8 from pUM3 & pBR322
RC   pUM12 from R773 & pBR322
RC   pUM300 series from pUM3 & F(Ts)::Tn5
RA   Chen C., Mobley H.L., Rosen B.P.;
RT   "Separate resistances to arsenate and arsenite (antimonate) encoded
RT   by the arsenical resistance operon of R factor R773";
RL   J. Bacteriol. 161:758-763(1985).
RN   [7]
RC   [pGP704 from M13tg131 & RP4 & R6K, ori]
RC   pJM703 from pRK703
RC   pJM703.1 from pJM703 & pSUP201-1
RC   pVM55 from pJM703.1 & pVM7
RA   Miller V.L., Mekalanos J.J.;
RT   "A novel suicide vector and its use in construction of insertion
RT   mutations: osmoregulation of outer membrane proteins and
RT   virulence determinants in Vibrio cholerae requires toxR";
RL   J. Bacteriol. 170:2575-2583(1988).
RN   [8]
RC   pTet1, pTet2 from pBR322 & M13 & pGC1 & Tn5 IS50R tnp gene
RA   Dodson K.W., Berg D.E.;
RT   "Factors affecting transposition activity of IS50 and Tn5 ends";
RL   Gene 76:207-213(1989).
RN   [9]
RC   pUM1 from pBR322 & R773, ars genes
RC   pUM3 from pBR322 & R773, ars genes
RA   Mobley H.L., Chem C.M., Silver S., Rosen B.P.;
RT   "Cloning and expression of R-factor mediated arsenate resistance in
RT   Escherichia coli";
RL   Mol. Gen. Genet. 191:421-426(1983).
RN   [10]
RC   R773 from ars genes
RA   Hedges R.W., Baumberg S.;
RT   "Resistance to arsenic compounds conferred by a plasmid
RT   transmissible between strains of Escherichia coli";
RL   J. Bacteriol. 115:459-460(1973).
RN   [11]
RC   F(Ts) from F factor
RC   F(Ts)::Tn5 from F(Ts) & Tn5
RA   Kaper J.;
RT   ;
RL   Unpublished (1985).
RN   [12]
RC   ColV from E. coli
RC   [ColV-K30 from E. coli]
RA   Bagg A., Neilands J.B.;
RT   "Mapping of a mutation affecting regulation of iron uptake systems
RT   in Escherichia coli K-12";
RL   J. Bacteriol. 161:450-453(1985).
RN   [13]
RC   pABN40 from ColV-K30
RA   Bindereif A., Neilands J.B.;
RT   "Promoter mapping and transcriptional regulation of the iron
RT   assimilation system of plasmid ColV-K30 in Escherichia coli K-12";
RL   J. Bacteriol. 162:1039-1046(1985).
RN   [14]
RC   pABN15 from ColV-K30
RA   De Lorenzo V., Bindereif A., Paw B., Neilands J.B.;
RT   "Aerobactin biosynthesis and transport genes of plasmid ColV-K30
RT   in Escherichia coli K-12";
RL   J. Bacteriol. 165:570-578(1986).
RN   [15]
RC   pVM7 from toxR gene
RA   Miller V.L., Mekalanos J.J.;
RT   "Synthesis of cholera toxin is positively regulated at the
RT   transcriptional level by toxR";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:3471-3475(1984).
RN   [16]
RC   pAG120 from Vibrio harveyi luxA/luxB genes
RA   Mileham A.J.;
RT   ;
RL   Unpublished (1987).
RN   [17]
RC   pC77/2 from pUC9 & pAG120
RC   pC86/2 from pTTQ19 & pAG120
RA   Plastow G.S.;
RT   ;
RL   Unpublished (1987).
RN   [18]
RC   pBG3, pBG4 from Streptomyces hygroscopicus bar gene
RA   Murakami T., Anzai H., Imai S., Satoh A., Nagaoka K., Thompson C.J.;
RT   ;
RL   Mol. Gen. Genet. 205:42-50(1986).
RN   [19]
RC   pDU1358 from Serratia marcescens incC plasmid
RA   Griffin H.G., Foster T.J., Falkiner F.R., Carr M.E., Coleman D.C.;
RT   "Molecular analysis of multiple-resistance plasmids transferred
RT   from gram-negative bacteria isolated in a urological unit";
RL   Antimicrob. Agents Chemother. 28:413-418(1985).
RN   [20]
RC   R831 from E. coli, merB gene
RA   Ogawa H.I., Tolle C.L., Summers A.O.;
RT   "Physical and genetic map of the organomercury resistance (Omr)
RT   and inorganic mercury resistance (Hgr) loci of the IncM plasmid
RT   R831b";
RL   Gene 32:311-320(1984).
RN   [21]
RC   pDU202 from R100.1 & tet gene
RA   Foster T.J., Willetts N.S.;
RT   ;
RL   Mol. Gen. Genet. 156:107-114(1977).
RN   [22]
RC   pDU1205 from R100, mer gene
RA   Foster T.J., Brown N.L.;
RT   "Identification of the merR gene of R100 by using mer-lac gene and
RT   operon fusions";
RL   J. Bacteriol. 163:1153-1157(1985).
RN   [23]
RC   pDU1003 from pBR322 & R100
RA   Ni'Bhriain N.N., Silver S., Foster T.J.;
RT   "Tn5 insertion mutations in the mercuric ion resistance genes
RT   derived from plasmid R100";
RL   J. Bacteriol. 155:690-703(1983).
CC   oligonucleotide adaptor.
CC   NM (pGP704)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC18)(R6K)(lambda 1105)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. M13tg131, MCS/amp gene
FT                   2. R6K, ori
FT                   3. RP4, mob gene
FT                   -> pGP704"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;