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ID   PGPTVKAN   preliminary; circular DNA; SYN; 13600 BP.
AC   ATCC77388;
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Higher plants/Agrobacterium/E.coli plasmid vector pGPTV-KAN - incomp.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   [pGPTV from pBIB-HYG & pBIN19 & pBI101.1]
RC   pGPTV-KAN from pGPTV & pRT100neo, neo gene
RC   pGPTV-HPT from pGPTV & hpt gene
RC   pGPTV-DHFR from pGPTV & DHFR gene
RC   pGPTV-BAR from pGPTV & bar gene
RC   pGPTV-BLEO from pGPTV & ble gene
RA   Becker D., Kemper E., Schell J., Masterson R.;
RT   "New plant binary vectors with selectable markers located proximal
RT   to the left T-DNA border";
RL   Plant Mol. Biol. 20:1195-1197(1992).
RN   [2]
RC   pGV3850::Ds-DHFR from pGV3850 & DHFR gene
RA   Kemper E., Grevelding C., Schell J., Masterson R.;
RT   "Improved method for the transformation of Arabidopsis thaliana with
RT   chimeric dihydrofolate reductase constructs which confer
RT   methotrexate resistance";
RL   Plant Cell Rep. 11:118-121(1992).
RN   [3]
RC   plasmid from pBI101.1 & pBIN19
RC   pBIG-KAN from plasmid & pPCV5013 Hyg & pBI101.1
RC   pBIG-HYG from plasmid & pPCV5013 Hyg & pGDW11
RC   pBIB-KAN from pUC19 & pBIG-KAN
RC   pBIB-HYG from pUC19 & pBIG-HYG
RA   Becker D.;
RT   "Binary vectors which allow the exchange of plant selectable markers
RT   and reporter genes";
RL   Nucleic Acids Res. 18:203-203(1990).
RN   [4]
RC   pRT100neo from CaMV 35S promoter & neo gene
RC   pRT101cat from CaMV 35S promoter & cat gene
RC   pCAP212 from P1/P2 promoter & cat gene
RC   pLGV1103neo from NOS promoter & cat gene
RC   pWDVneo1, pWDVneo2 from WDV promoter & cat gene
RA   Topfer R., Gronenborn B., Schell J., Steinbiss H.H.;
RT   "Uptake and transient expression of chimeric genes in seed-derived
RT   embryos";
RL   Plant Cell 1:133-139(1989).
RN   [5]
RC   pPCV5013 Hyg from NOS promoter
RA   Koncz C., Martini N., Mayerhofer R., Koncz-Kalman Z., Korber H.,
RA   Redei G.P., Schell J.;
RT   "High-frequency T-DNA-mediated gene tagging in plants";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:8467-8471(1990).
CC   Deposited by: Robert Masterson, D. Becker
CC   Binary vector with plant selectable marker near the T-DNA left border
CC   and a glucuronidase (uidA) reporter gene.  The marker and uidA are
CC   divergently transcribed, and can be easily removed or replaced. [1]
CC   The order of the major features of this plasmid is: EcoRI - pAnos -
CC   SstI - uidA - SmaI - XbaI - SalI - HindIII - Pnos - BglII - nptII -
CC   BamHI - pAg7- T-DNA - BglII - RK2 ori - kanR. [1]
CC   Vector contains the following restriction sites (approximate kb from
CC   the HindIII site): BamHI--1.4; EcoRI--11.4; SstI--11.7. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--13.6; BglII--8.2, 3.2, 2.1; HindIII--13.6. (ATCC staff)
CC   Medium is 1236 LB plus kanamycin.
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(E.coli HB101)(Agrobacterium tumefaciens)(higher plants)
CC   HO (Agrobacterium tumefaciens LBA4404)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Ti, nos promoter
FT                   2. E. coli 1142bp, hyg gene
FT                   -> pPCV5013 Hyg
FT                   1. pPCV5013 Hyg
FT                   2. pGDW11 1150bp, hyg gene
FT                   -> plasmid
FT                   1. pBI101.1 BamHI 5350bp 2529..2529
FT                   Klenow
FT                   HindIII-SstII/SacII 3033bp 2499..5350..182
FT                   blunt end:
FT                   2. pBIN19 EcoRI-BamHI 21bp 6773..6794, gene 7 polyA
FT                   blunt end:
FT                   3. plasmid BamHI-HindIII, nos promoter/hyg gene
FT                   -> pBIG-HYG
FT                   1. pBIN19 BamHI 11777bp 6794..6794, MCS
FT                   fill in
FT                   HindIII-SstI/SacII 2323bp 6824..9147,
FT                   \ MCS/may be SacII 93
FT                   2. pBIG-HYG remove HindIII-SstI/SacII 3033bp
FT                   \ 2499..5350..182, GUS gene/ bp
FT                   -> pBIB-HYG
FT                   1. pBIB-HYG 1150bp, hyg gene
FT                   2. pBIN19, ori
FT                   3. pBI101.1, GUS gene
FT                   -> pGPTV 12000bp
FT                   1. pRT100 3340bp, CaMV 35S promoter
FT                   2. Tn5 BglII-SalI 1169bp 1516..2685, neo/kan gene
FT                   -> pRT100neo 4500bp
FT                   1. pGPTV BamHI 12000bp
FT                   2. pRT100neo BglII-BglII 1600bp 1516..?, neo/kan gene
FT                   -> pGPTV-KAN 13600bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SstI-SmaI-XbaI-SalI-
FT                   HindIII-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;