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ID   PGPTVKAN   preliminary; circular DNA; SYN; 13600 BP.
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AC   ATCC77388;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Higher plants/Agrobacterium/E.coli plasmid vector pGPTV-KAN - incomp.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pGPTV from pBIB-HYG & pBIN19 & pBI101.1]
RC   pGPTV-KAN from pGPTV & pRT100neo, neo gene
RC   pGPTV-HPT from pGPTV & hpt gene
RC   pGPTV-DHFR from pGPTV & DHFR gene
RC   pGPTV-BAR from pGPTV & bar gene
RC   pGPTV-BLEO from pGPTV & ble gene
RA   Becker D., Kemper E., Schell J., Masterson R.;
RT   "New plant binary vectors with selectable markers located proximal
RT   to the left T-DNA border";
RL   Plant Mol. Biol. 20:1195-1197(1992).
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RN   [2]
RC   pGV3850::Ds-DHFR from pGV3850 & DHFR gene
RA   Kemper E., Grevelding C., Schell J., Masterson R.;
RT   "Improved method for the transformation of Arabidopsis thaliana with
RT   chimeric dihydrofolate reductase constructs which confer
RT   methotrexate resistance";
RL   Plant Cell Rep. 11:118-121(1992).
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RN   [3]
RC   plasmid from pBI101.1 & pBIN19
RC   pBIG-KAN from plasmid & pPCV5013 Hyg & pBI101.1
RC   pBIG-HYG from plasmid & pPCV5013 Hyg & pGDW11
RC   pBIB-KAN from pUC19 & pBIG-KAN
RC   pBIB-HYG from pUC19 & pBIG-HYG
RA   Becker D.;
RT   "Binary vectors which allow the exchange of plant selectable markers
RT   and reporter genes";
RL   Nucleic Acids Res. 18:203-203(1990).
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RN   [4]
RC   pRT100neo from CaMV 35S promoter & neo gene
RC   pRT101cat from CaMV 35S promoter & cat gene
RC   pCAP212 from P1/P2 promoter & cat gene
RC   pLGV1103neo from NOS promoter & cat gene
RC   pWDVneo1, pWDVneo2 from WDV promoter & cat gene
RA   Topfer R., Gronenborn B., Schell J., Steinbiss H.H.;
RT   "Uptake and transient expression of chimeric genes in seed-derived
RT   embryos";
RL   Plant Cell 1:133-139(1989).
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RN   [5]
RC   pPCV5013 Hyg from NOS promoter
RA   Koncz C., Martini N., Mayerhofer R., Koncz-Kalman Z., Korber H.,
RA   Redei G.P., Schell J.;
RT   "High-frequency T-DNA-mediated gene tagging in plants";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:8467-8471(1990).
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CC   Deposited by: Robert Masterson, D. Becker
CC   Binary vector with plant selectable marker near the T-DNA left border
CC   and a glucuronidase (uidA) reporter gene.  The marker and uidA are
CC   divergently transcribed, and can be easily removed or replaced. [1]
CC   The order of the major features of this plasmid is: EcoRI - pAnos -
CC   SstI - uidA - SmaI - XbaI - SalI - HindIII - Pnos - BglII - nptII -
CC   BamHI - pAg7- T-DNA - BglII - RK2 ori - kanR. [1]
CC   Vector contains the following restriction sites (approximate kb from
CC   the HindIII site): BamHI--1.4; EcoRI--11.4; SstI--11.7. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--13.6; BglII--8.2, 3.2, 2.1; HindIII--13.6. (ATCC staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pGPTV-KAN)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli HB101)(Agrobacterium tumefaciens)(higher plants)
CC   HO (Agrobacterium tumefaciens LBA4404)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Ti, nos promoter
FT                   2. E. coli 1142bp, hyg gene
FT                   -> pPCV5013 Hyg
FT                   1. pPCV5013 Hyg
FT                   2. pGDW11 1150bp, hyg gene
FT                   -> plasmid
FT                   1. pBI101.1 BamHI 5350bp 2529..2529
FT                   Klenow
FT                   HindIII-SstII/SacII 3033bp 2499..5350..182
FT                   blunt end:
FT                   2. pBIN19 EcoRI-BamHI 21bp 6773..6794, gene 7 polyA
FT                   blunt end:
FT                   3. plasmid BamHI-HindIII, nos promoter/hyg gene
FT                   -> pBIG-HYG
FT                   1. pBIN19 BamHI 11777bp 6794..6794, MCS
FT                   fill in
FT                   HindIII-SstI/SacII 2323bp 6824..9147,
FT                   \ MCS/may be SacII 93
FT                   2. pBIG-HYG remove HindIII-SstI/SacII 3033bp
FT                   \ 2499..5350..182, GUS gene/ bp
FT                   -> pBIB-HYG
FT                   1. pBIB-HYG 1150bp, hyg gene
FT                   2. pBIN19, ori
FT                   3. pBI101.1, GUS gene
FT                   -> pGPTV 12000bp
FT                   1. pRT100 3340bp, CaMV 35S promoter
FT                   2. Tn5 BglII-SalI 1169bp 1516..2685, neo/kan gene
FT                   -> pRT100neo 4500bp
FT                   1. pGPTV BamHI 12000bp
FT                   2. pRT100neo BglII-BglII 1600bp 1516..?, neo/kan gene
FT                   -> pGPTV-KAN 13600bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SstI-SmaI-XbaI-SalI-
FT                   HindIII-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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