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ID   PGR102     preliminary; circular DNA; SYN; 328 BP.
AC   M25299;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pGR102 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-328
RC   pWG29delta3 from pWG29
RC   pRSV-MMTV from pGR102 & pRSV-2, RSV LTR
RC   RMC2h, RMC6h from pRSV-MMTV & pY3
RA   Salmons B., Moritz-Legrand S., Garcha I., Guenzburg W.H.;
RT   "Construction and characterization of a packaging cell line for
RT   MMTV-based conditional retroviral vectors";
RL   Biochem. Biophys. Res. Commun. 159:1191-1198(1989).
RN   [2]
RC   pWG29 from MMTV
RA   Gunzburg W.H., Salmons B.;
RT   "Mouse mammary tumor virus mediated transfer and expression of
RT   neomycin resistance to infected cultured cells";
RL   Virology 155:236-248(1986).
RN   [3]
RC   pGR102 from MMTV
RA   Salmons B., Groner B., Calberg Bacq C.M., Ponta H.;
RT   "Production of mouse mammary tumor virus upon transfection of a
RT   recombinant proviral DNA into cultured cells";
RL   Virology 144:101-114(1985).
RN   [4]
RC   pRSV-2 from RSV LTR
RA   Gorman C.;
RT   ;
RL   Unpublished (1989).
CC   NCBI gi: 341326
CC   NM (pGR102)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(vertebrate)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. MMTV, gag gene
FT                   2. RSV, U3/R/U5
FT                   -> pGR102"
FT   LTR             <1..6
FT                   /note="RSV U3 region"
FT   LTR             7..21
FT                   /note="RSV R region"
FT   LTR             22..141
FT                   /note="RSV U5 region"
FT   primer_bind     142..159
FT                   /note="primer"
FT   RBS             292..295
FT                   /note="RBS MMTV gag gene"
FT   CDS             318..>328
FT                   /note="MMTV gag gene; packaging"
SQ   Sequence 328 BP; 59 A; 97 C; 82 G; 90 T; 0 other;
     aaacttgcaa cagtcctaac attcacctct cgtgtgtttg tgtctgttcg ccatcccgtc
     tccgctcgtc acttatcctt cactttccag agggtccccc cgcagacccc ggtgaccctc
     aggtcagccg actgcggcag ctggcgcccg aacagggccc tcggataagt gacccttgtc
     tctatttcta ctatttggtg ttcgtcttgt attgtctctt tcttgtctgg ctatcatcac
     aagagcggaa cggactcacc atagggagct gcagtcccgc ctacggagaa gaggtaggtt
     atggtgagcc attggatatg ggggtctc