back Return to this vector's summary.
ID   PGR71      preliminary; circular DNA; SYN; 8300 BP.
XX
AC   ATCC37105;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/E.coli plasmid vector pGR71 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBR350 from pBR322
RC   pEB1 from pBR328 & pUB110
RC   pCM71 from pCM1 & pCM7
RC   pGR1 from pUB110 & pBR350
RC   pGR71 from pGR1 & pCM71
RC   pGR71- series from pGR71
RA   Goldfarb D.S., Doi R.H., Rodriguez R.L.;
RT   "Expression of Tn9-derived chloramphenicol resistance in Bacillus
RT   subtilis";
RL   Nature 293:309-311(1981).
XX
CC   Chloramphenicol resistance is low in E.coli.
CC   A vector for cloning Bacillus subtilis expression regulatory elements.
CC   (ATCC staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pGR71)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Bacillus subtilis)(E.coli)
CC   HO (Bacillus subtilis BD170)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove 600bp, amp gene/3760bp
FT                   -> pBR350 3760bp
FT                   1. pUB110 EcoRI 4548bp 2..2
FT                   2. pBR350 EcoRI 3760bp, 5' to tet gene/pBR322 4360
FT                   -> pGR1 8310bp
FT                   1. pGR1 remove HindIII-SalI 622bp, tet gene
FT                   \ pBR322 HindIII-SalI 30..652/7590bp
FT                   2. pCM71 HindIII-SalI 725bp 3115..3370..470, cat gene
FT                   \ [786bp]
FT                   -> pGR71 8315bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
XX
SQ   Sequence 44 BP; 16 A; 6 C; 13 G; 9 T; 0 other;
     aagcttcgac gagattttca ggagctaagg aagctaaaat ggag
//