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ID   PGRE51     preliminary; circular DNA; SYN; 4000 BP.
AC   IG5201;
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pGRE5-1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   GRE/pAL10, GRE2/pAL10, GRE5/pAL10 from pAL10 & oligo, rat TAT GRE
RC   pGRE5-1, pGRE5-2 from GRE5/pAL10 & linker
RC   pGRE5/CAT from pGRE5-1 & pBLCAT8+
RC   pGRE1tkCAT from pBLCAT8+ & oligo, rat TAT GRE
RC   pGRE2tkCAT from pBLCAT8+ & oligo, rat TAT GRE
RC   p220.2-GRE5/CAT from pGRE5/CAT & p220.2
RA   Mader S., White J.H.;
RT   "A steroid-inducible promoter for the controlled overexpression
RT   of cloned genes in eukaryotic cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:5603-5607(1993).
RN   [2]
RC   pGRE5/CAT from pGRE5-1 & pBLCAT8+
RA   Mader S., White J.H.;
RT   ;
RL   Comments 20:57-62(1993).
RN   [3]
RC   pRX2 from adenovirus 2 MLP TATA
RC   pAL4 from pBluescript M13+ & rabbit beta-globin gene & SV40 terminator
RC   pAL10 from pAL4 & pRX2, adenovirus 2 MLP TATA
RC   pERE10, pERE11, pERE12, pERE100 series from pAL10 & ERE
RC   pAL13 from pAL10 & oligo
RC   pERE20, pERE200 series from pAL10 & ERE
RC   pERE300 series from pAL10 & ERE
RA   Ponglikitmongkol M., White J.H., Chambon P.;
RT   "Synergistic activation of transcription by the human estrogen
RT   receptor bound to tandem responsive elements";
RL   EMBO J. 9:2221-2231(1990).
RN   [4]
RC   HE series from BlueScribe M13+ & human ER gene
RC   HE series from M13mp18 & human ER gene
RC   from pKCR2 & HE series
RC   HG0 from pKCR2 & human GR gene
RC   HG1 from pKCR2 & human GR gene
RC   HG3 from HG1 & BlueScribe M13+
RC   pS2-CAT from pCOL-CAT & pS2 promoter
RC   vit-tk-globin from vit-tk-CAT & pA0
RC   pS2-globin from pS2 gene & pA0
RA   Kumar V., Green S., Stack G., Berry M., Jin J.R., Chambon P.;
RT   "Functional domains of the human estrogen receptor";
RL   Cell 51:941-951(1987).
RN   [5]
RC   vit-tk-CAT from chicken vitellogenin gene/tk gene & cat gene
RA   Klein-Hitpass L., Schorpp M., Wagner U., Ryffel G.U.;
RT   "An estrogen-responsive element derived from the 5' flanking region
RT   of the Xenopus vitellogenin A2 gene functions in transfected human
RT   cells";
RL   Cell 48:1053-1061(1986).
RN   [6]
RC   MMTV-CAT from MMTV & cat gene
RA   Cato A.C., Miksicek R., Schutz G., Arnemann J., Beato M.;
RT   "The hormone regulatory element of mouse mammary tumour virus
RT   mediates progesterone induction";
RL   EMBO J. 5:2237-2240(1986).
RN   [7]
RC   MMTV-globin from MMTV & globin gene
RA   Gronemeyer H., Turcotte B., Quinn-Stricker C., Bocquel M.T.,
RA   Meyer M.E., Krozowski Z., Jeltsch J.M., Lerouge T., Garnier J.M.,
RA   Chambon P.;
RT   "The chicken progesterone receptor: sequence, expression and
RT   functional analysis";
RL   EMBO J. 6:3985-3994(1987).
RN   [8]
RC   pA0 from globin gene
RA   Zenke M., Grundstrom T., Matthes H., Wintzerith M., Schatz C.,
RA   Wildeman A., Chambon P.;
RT   "Multiple sequence motifs are involved in SV40 enhancer function";
RL   EMBO J. 5:387-397(1986).
CC   Plasmid to be cotransfected with plasmids encoding steroid receptors
CC   such as pRShGR (ATCC 67200) and pRShMR (ATCC 67201) to provide a
CC   reporter gene for hormone-dependent transcriptional activation. [3]
CC   Contains the LTR from mouse mammary tumor virus (nt -142 to nt -109),
CC   sequences encoding chloramphenicol acetyltransferase, and SV40
CC   splicing sequences. [3]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--4.2, 1.8; PvuII--5.95, 0.3; BglI--4.8, 1.2; HindIII--3.55,
CC   2.45; PstI--6.0. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pGRE5-1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(eukaryote cells)
CC   CP ()
CC   FN (cloning)(expression eukaryote)
CC   SE ()
CC   PA ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. adenovirus 2 MLP TATA
FT                   -> pRX2
FT                   1. Bluescribe M13+ remove KpnI-HindIII 35bp 898..933,
FT                   \ MCS 3169bp
FT                   2. pAO BamHI 1559bp,
FT                   \ rabbit beta-globin gene -9 to +1550
FT                   in vitro mutagenesis
FT                   BglII 1559bp, rabbit beta-globin gene -9 to +1550
FT                   in vitro mutagenesis
FT                   1559bp, rabbit beta-globin gene -9 to +1550
FT                   -> plasmid 4728bp
FT                   1. plasmid 4728bp
FT                   2. SV40 KpnI-HindIII 748bp 299..1047, terminator
FT                   -> pAL4 5476bp
FT                   1. pAL4 remove KpnI-BglII, MCS
FT                   \ Bluescribe M13+ KpnI 898/no BglII in SV40 or Blue..
FT                   2. pRX2 KpnI-BamHI, adenovirus 2 MLP TATA -95 to +33
FT                   -> pAL10
FT                   1. pAL10 BglII, -65
FT                   2. oligo BamHI-rat TAT GRE-BglII
FT                   3. oligo BamHI-rat TAT GRE-BglII
FT                   4. oligo BamHI-rat TAT GRE-BglII
FT                   5. oligo BamHI-rat TAT GRE-BglII
FT                   6. oligo BamHI-rat TAT GRE-BglII
FT                   -> GRE5/pAL10 4000bp
FT                   1. GRE5/pAL10 remove small EcoRI-BglII, 4000bp
FT                   2. linker EcoRI-KpnI-SmaI-SacI-XhoI-HindIII-BglII
FT                   -> pGRE5-1 4000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   promoter        0..0
FT                   /note="PRO eukaryote glucocorticoid response elements
FT                   GRE (five)"
FT   promoter        0..0
FT                   /note="PRO adenovirus 2 major late genes TATA region"
FT   misc_feature    0..0
FT                   /note="rabbit beta-globin intron"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-KpnI-SmaI-SacI-XhoI-HindIII-BglII"
FT   polyA_signal    0..0
FT                   /note="PLA SV40"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;