back Return to this vector's summary.
ID   PGRE52     preliminary; circular DNA; SYN; 4000 BP.
XX
AC   IG5202;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pGRE5-2 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   GRE/pAL10, GRE2/pAL10, GRE5/pAL10 from pAL10 & oligo, rat TAT GRE
RC   pGRE5-1, pGRE5-2 from GRE5/pAL10 & linker
RC   pGRE5/CAT from pGRE5-1 & pBLCAT8+
RC   pGRE1tkCAT from pBLCAT8+ & oligo, rat TAT GRE
RC   pGRE2tkCAT from pBLCAT8+ & oligo, rat TAT GRE
RC   p220.2-GRE5/CAT from pGRE5/CAT & p220.2
RA   Mader S., White J.H.;
RT   "A steroid-inducible promoter for the controlled overexpression
RT   of cloned genes in eukaryotic cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:5603-5607(1993).
XX
CC   NM (pGRE5-2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(eukaryote cells)
CC   CP ()
CC   FN (cloning)(expression eukaryote)
CC   SE ()
CC   PA ()
CC   BR (pGRE5-1)(pGRE5CAT)(pGRE5-1EBV)(pGRE5-2EBV)(pGRE5CATEBV)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. adenovirus 2 MLP TATA
FT                   -> pRX2
FT                   1. Bluescribe M13+ remove KpnI-HindIII 35bp 898..933,
FT                   \ MCS 3169bp
FT                   2. pAO BamHI 1559bp,
FT                   \ rabbit beta-globin gene -9 to +1550
FT                   in vitro mutagenesis
FT                   BglII 1559bp, rabbit beta-globin gene -9 to +1550
FT                   in vitro mutagenesis
FT                   1559bp, rabbit beta-globin gene -9 to +1550
FT                   -> plasmid 4728bp
FT                   1. plasmid 4728bp
FT                   2. SV40 KpnI-HindIII 748bp 299..1047, terminator
FT                   -> pAL4 5476bp
FT                   1. pAL4 remove KpnI-BglII, MCS
FT                   \ Bluescribe M13+ KpnI 898/no BglII in SV40 or Blue..
FT                   2. pRX2 KpnI-BamHI, adenovirus 2 MLP TATA -95 to +33
FT                   -> pAL10
FT                   1. pAL10 BglII, -65
FT                   2. oligo BamHI-rat TAT GRE-BglII
FT                   3. oligo BamHI-rat TAT GRE-BglII
FT                   4. oligo BamHI-rat TAT GRE-BglII
FT                   5. oligo BamHI-rat TAT GRE-BglII
FT                   6. oligo BamHI-rat TAT GRE-BglII
FT                   -> GRE5/pAL10 4000bp
FT                   1. GRE5/pAL10 remove small EcoRI-BglII, 4000bp
FT                   2. linker BglII-HindIII-XhoI-SacI-SmaI-KpnI-EcoRI
FT                   -> pGRE5-2 4000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   promoter        0..0
FT                   /note="PRO eukaryote glucocorticoid response elements
FT                   GRE (five)"
FT   promoter        0..0
FT                   /note="PRO adenovirus 2 major late genes TATA region"
FT   misc_feature    0..0
FT                   /note="rabbit beta-globin intron"
FT   misc_binding    0..0
FT                   /note="MCS BglII-HindIII-XhoI-SacI-SmaI-KpnI-EcoRI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//