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ID   PGRE5CTEBV preliminary; circular DNA; SYN; 10100 BP.
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AC   IG5206;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pGRE5/CAT/EBV - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   GRE/pAL10, GRE2/pAL10, GRE5/pAL10 from pAL10 & oligo, rat TAT GRE
RC   pGRE5-1, pGRE5-2 from GRE5/pAL10 & linker
RC   pGRE5/CAT from pGRE5-1 & pBLCAT8+
RC   pGRE1tkCAT from pBLCAT8+ & oligo, rat TAT GRE
RC   pGRE2tkCAT from pBLCAT8+ & oligo, rat TAT GRE
RC   p220.2-GRE5/CAT from pGRE5/CAT & p220.2
RA   Mader S., White J.H.;
RT   "A steroid-inducible promoter for the controlled overexpression
RT   of cloned genes in eukaryotic cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:5603-5607(1993).
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CC   NM (pGRE5CATEBV)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(primate cells)
CC   CP ()
CC   FN (cloning)(expression eukaryote)
CC   SE ()
CC   PA ()
CC   BR (pGRE5-1)(pGRE5-2)(pGRE5CAT)(pGRE5-1EBV)(pGRE5-2EBV)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGRE5/CAT 4000bp
FT                   2. EBV 6100bp, ori/EBNA-1 gene
FT                   -> pGRE5CATEBV 10100bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   promoter        0..0
FT                   /note="PRO eukaryote glucocorticoid response elements
FT                   GRE (five)"
FT   promoter        0..0
FT                   /note="PRO adenovirus 2 major late genes TATA region"
FT   misc_feature    0..0
FT                   /note="rabbit beta-globin intron"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   misc_binding    0..0
FT                   /note="MCS BglII-HindIII-XhoI-SacI-SmaI-KpnI-EcoRI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   CDS             0..0
FT                   /note="ANT mammal hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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