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ID   PGS3       preliminary; circular DNA; SYN; 7800 BP.
AC   ATCC37184;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pGS3 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pKO482 from galK gene
RC   pML-galK from pML2 & linker & pKO482, galK gene
RC   pGS3 from pML-galK & SV40 early region/ori
RA   Razzaque A., Mizusawa H., Seidman M.M.;
RT   "Rearrangement and mutagenesis of a shuttle vector plasmid after
RT   passage in mammalian cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:3010-3014(1983).
RN   [2]
RC   pCa series from pBR322 & African Green monkey alpha-satellite DNA
RC   pCa10, pCa18, pCa1003, pCa1004 from pBR322 & alpha-satellite DNA
RC   pCa119, pCa85, pCa329, pCa1005 from pBR322 & alpha-satellite DNA
RA   Thayer R.E., Singer M.F., McCutchan T.F.;
RT   "Sequence relationships between single repeat units of highly
RT   reiterated African Green monkey DNA";
RL   Nucleic Acids Res. 9:169-181(1981).
CC   An E.coli/mammalian cell culture shuttle vector. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pGS3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   HO (R 1610 cell line [ATCC CRL 1657])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. E. coli, galK gene
FT                   -> pKO482
FT                   1. pML2 BamHI-AatII 2522bp 306..2828, [2523bp]
FT                   2. pKO482 AccI
FT                   BAL31 nuclease, remove 200bp
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   EcoRI-BamHI 2200bp, galK gene
FT                   -> pML-galK 4700bp
FT                   1. pML-galK BamHI 4700bp 0..0
FT                   2. SV40 BamHI-HpaII 3056bp 2534..5243..347,
FT                   \ early region/ori
FT                   BamHI linker 6bp ggatcc:
FT                   BamHI-BamHI 3060bp, early region/ori
FT                   -> pGS3 7800bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   CDS             0..0
FT                   /note="GEN SV40 large T antigen gene"
FT   CDS             0..0
FT                   /note="GEN SV40 small T antigen gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;