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ID   PGSR100    preliminary; circular DNA; SYN; 8000 BP.
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AC   IG7015;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pGSR100 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBR322-gshI from pBR322 & E.coli gshI gene
RC   [pGS100 from E. coli gshI gene]
RC   [pGSR100 from pGS100, E. coli gshI gene]
RA   Murata K., Kimura A.;
RT   "Cloning of a gene responsible for the biosynthesis of glutathione
RT   in Escherichia coli B";
RL   Appl. Environ. Microbiol. 44:1444-1448(1982).
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CC   NM (pGSR100)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E. coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pGS100)
CC   BR ()
CC   OF (pGSR150 from pGSR100 and pGSR1)(pGSR151 from pGSR150)
CC   OF (pGSR1518 from pGSR151 and pMC1585 with P8 promoter)
CC   OF (pGSR2518 from pGSR1518)(pGSR2518-X from pGSR2518)
CC   OR (yes)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/tet gene
FT                   2. E. coli, gshI gene
FT                   -> pGS100
FT                   1. pGS100, E. coli gshI gene
FT                   -> pGSR100 8000bp"
FT   misc_feature    0..0
FT                   /note="PstI"
FT   misc_feature    0..0
FT                   /note="gshI"
FT   misc_feature    0..0
FT                   /note="HpaI"
FT   misc_feature    0..0
FT                   /note="HpaI"
FT   misc_feature    0..0
FT                   /note="PstI"
FT   misc_feature    0..0
FT                   /note="ScaI"
FT   misc_feature    0..0
FT                   /note="tet"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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