back Return to this vector's summary.
ID   PGV1106    preliminary; circular DNA; SYN; 10500 BP.
AC   ATCC37169;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli plasmid vector pGV1106 - incomplete.
KW   cloning vector; W plasmid.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pGV1106 from pSa
RC   pGV1113 from pGV1106 & R702, P plasmid with Su
RC   pGV1120 from pGV1113 & pBR325
RC   pGV1122 from pGV1120
RC   pGV1124 from pGV1113 & pACYC184
RA   Leemans J., Langenakens J., De Greve H., Deblaere R., Van Montagu M.,
RA   Schell J.;
RT   "Broad-host-range cloning vectors derived from the W-plasmid Sa";
RL   Gene 19:361-364(1982).
RN   [2]
RC   R934, R702, pUZ8 from E. coli P group plasmids
RC   R483 from E. coli Ialpha group plasmids
RC   RN3, R128 from E. coli N group plasmids
RA   Leemans J., Inze D., Villaroel R., Engler G., Hernalsteens J.P.,
RA   De Block M., Van Montagu M.;
RT   "Plasmid mobilization as a tool for in vivo genetic engineering";
RL   Molecular Biology Pathogen Ecol of Bacterial Plasmids 0:401-410(1981).
RL   Levy S.B., Clowes R.C., Koenig E.L., eds., Plenum, New York.
RN   [3]
RC   RN3, R128 from E. coli N group plasmids
RA   Grindley N.D., Grindley J.N., Anderson E.S.;
RT   "R factor compatibility groups";
RL   Mol. Gen. Genet. 119:287-297(1972).
CC   A broad host range plasmid vector. (ATCC staff)
CC   Nonconjugative.  Caution: this plasmid is readily lost from the
CC   E. coli host.  Always grow under antibiotic selection.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--10.5; EcoRI--10.5; PstI--10.5; BglII--10.5; BamHI/EcoRI--8.0,
CC   2.5; BamHI/BglII--6.2, 4.3. (ATCC staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pGV1106)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(broad host range)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pSa BglII-BglII 2200bp, kan/spec/strep
FT                   -> pGV1106 10500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-PstI-BglII-BamHI-SacII-KpnI"
FT   rep_origin      0..0
FT                   /note="ORI broad host range Sa W-plasmid "
FT   CDS             0..0
FT                   /note="ANT broad host range kanamycin resistance gene
FT                   (kan)"
FT   CDS             0..0
FT                   /note="ANT broad host range spectinomycin resistance
FT                   gene (spc)"
FT   CDS             0..0
FT                   /note="ANT broad host range streptomycin resistance
FT                   gene (str)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;