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ID   PGV1122    preliminary; circular DNA; SYN; 11000 BP.
AC   ATCC37171;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli plasmid vector pGV1122 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pGV1106 from pSa
RC   pGV1113 from pGV1106 & R702, P plasmid with Su
RC   pGV1120 from pGV1113 & pBR325
RC   pGV1122 from pGV1120
RC   pGV1124 from pGV1113 & pACYC184
RA   Leemans J., Langenakens J., De Greve H., Deblaere R., Van Montagu M.,
RA   Schell J.;
RT   "Broad-host-range cloning vectors derived from the W-plasmid Sa";
RL   Gene 19:361-364(1982).
CC   This is a nonconjugative plasmid.  It contains the bom site of ColE1.
CC   A broad host range plasmid vector. (ATCC staff)
CC   Medium is 1065 LB medium.
CC   NM (pGV1122)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli K-12 r-m-)(broad host range)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pGV1113 remove small PstI-HindIII
FT                   2. pBR325 HindIII-PstI 3582bp 30..3612, ori/tet gene
FT                   -> pGV1120 [no sulf]
FT                   1. pGV1120 remove small BamHI-BglII, pGV1113/11000bp
FT                   -> pGV1122 11000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-BamHI-PstI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI broad host range Sa W-plasmid "
FT   CDS             0..0
FT                   /note="ANT broad host range tetracycline resistance
FT                   gene (tet)"
FT   CDS             0..0
FT                   /note="ANT broad host range spectinomycin resistance
FT                   gene (spc)"
FT   CDS             0..0
FT                   /note="ANT broad host range streptomycin resistance
FT                   gene (str)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;