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ID   PGV1124    preliminary; circular DNA; SYN; 11000 BP.
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AC   ATCC37172;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli plasmid vector pGV1124 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGV1106 from pSa
RC   pGV1113 from pGV1106 & R702, P plasmid with Su
RC   pGV1120 from pGV1113 & pBR325
RC   pGV1122 from pGV1120
RC   pGV1124 from pGV1113 & pACYC184
RA   Leemans J., Langenakens J., De Greve H., Deblaere R., Van Montagu M.,
RA   Schell J.;
RT   "Broad-host-range cloning vectors derived from the W-plasmid Sa";
RL   Gene 19:361-364(1982).
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CC   A broad host range plasmid vector. (ATCC staff)
CC   Nonconjugative.
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pGV1124)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli K-12 r-m-)(broad host range)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGV1113 remove short HindIII-BamHI 400bp,
FT                   \ 10600bp
FT                   2. pACYC184 HindIII-BamHI 346bp 1525..1871, cat gene
FT                   -> pGV1124 11000bp [EcoRI in cat]"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-BamHI-SalI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI broad host range Sa W-plasmid "
FT   CDS             0..0
FT                   /note="ANT broad host range chloramphenicol
FT                   acetyltransferase gene (cat); chloramphenicol
FT                   resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT broad host range spectinomycin resistance
FT                   gene (spc)"
FT   CDS             0..0
FT                   /note="ANT broad host range streptomycin resistance
FT                   gene (str)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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