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ID   PGV403     preliminary; circular DNA; SYN; 1 BP.
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AC   IG8060;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pGV403 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGV001 from pBR327
RC   pCSV03, pCSV27 from pGV001 & linker
RC   pCSV31 from pCSV03 & linker
RC   [pGV401, pGV403 from pCSV31]
RA   Volckaert G., DeVleeschouwer E., Blocker H., Frank R.;
RT   "A novel type of cloning vectors for ultrarapid chemical degradation
RT   sequencing of DNA";
RL   Gene Anal. Tech. 1:52-59(1984).
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CC   NM (pGV403)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Amersham)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pCVS31)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR327 HindIII-AvaI 1878bp 1425..3273..30
FT                   Klenow:Klenow
FT                   -> pGV001 1882bp [HindIII, no AvaI]
FT                   1. pGV001 ClaI 1882bp 25..25
FT                   Klenow with CG only
FT                   2. SmaI-BstEII linker 10bp gtgacccggg
FT                   -> pCSV03 1894bp
FT                   1. pCSV03 SmaI 1894bp 25..25
FT                   2. linker Tth111I-Tth111I 18bp gactgagtcgacttagtc
FT                   -> pCSV31 1912bp
FT                   1. pCSV31 1912bp
FT                   -> pGV403 1900bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//