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ID   PHD        preliminary; circular DNA; SYN; 4200 BP.
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AC   X12782;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E.coli plasmid vector pHD - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pTK-CAT from cat gene & tk gene
RC   pHD from pTK-CAT
RC   pHDMT4 from pHD & pMT4
RC   pHDCAT from pHD & cat gene
RA   Muller G., Ruppert S., Schmid E., Schutz G.;
RT   "Functional analysis of alternatively spliced tyrosinase gene
RT   transcripts";
RL   EMBO J. 7:2723-2730(1988).
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RN   [2]
RC   pMT4, pMT5 from mouse melanoma DNA & pcDV1 & pL1
RC   pKCRH2MT from pMT4 & pKCRH2
RA   Shibahara S., Tomita Y., Sakakura T., Nager C., Chaudhuri B.,
RA   Muller R.;
RT   "Cloning and expression of cDNA encoding mouse tyrosinase";
RL   Nucleic Acids Res. 14:2413-2427(1986).
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RN   [3]
RC   pKCRH2 from SV40
RA   Mishina M., Kurosaki T., Tobimatsu T., Morimoto Y., Noda M.,
RA   Yamamoto T., Terao M., Lindstrom J., Takahashi T., Kuno M., Numa S.;
RT   "Expression of functional acetylcholine receptor from cloned
RT   cDNAs";
RL   Nature 307:604-608(1984).
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CC   NM (pHD)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. HSV, tk gene/#X14112
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   3. pBR322
FT                   -> pTK-CAT
FT                   1. pTK-CAT
FT                   2. SV40 72bp 179..250,
FT                   \ 72bp repeat/5' to HSV tk promoter
FT                   -> pTK-CAT-14C
FT                   1. pTK-CAT-14C
FT                   2. MCS linker PstI-BglII-XhoI-KpnI-SmaI-BamHI-
FT                   EcoRV-HindIII-ClaI
FT                   -> pHD 4200bp"
FT   misc_feature    0..0
FT                   /note="SV40 72 bp repeats (4)"
FT   promoter        0..0
FT                   /note="PRO HSV tk gene"
FT   misc_binding    0..0
FT                   /note="MCS unique PstI-BglII-XhoI-KpnI-SmaI-BamHI-
FT                   EcoRV-HindIII-ClaI"
FT   misc_feature    0..0
FT                   /note="SV40 splicing sequences"
FT   polyA_signal    0..0
FT                   /note="PLA SV40"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO A. tumefaciens T-DNA gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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