back Return to this vector's summary.
ID   PHEBO1     preliminary; circular DNA; SYN; 6900 BP.
XX
AC   ATCC39820;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pHEBo1 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pHEBo1 from pBR322 & EBV oriP & HSV tk promoter & hyg gene
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,686,186 dated Aug. 11, 1987.
XX
RN   [2]
RC   pHEBo1 from pBR322 & EBV oriP & HSV tk promoter & hyg gene
RA   Maniatis T., Fritsch E., Sambrook J.;
RT   ;
RL   Molecular Cloning 0:16-27(1989).
RL   Maniatis T., Fritsch E., Sambrook J.,
RL   2nd ed., Cold Spring Harbor Laboratory, New York.
XX
CC   A shuttle vector which can replicate and be selected in E.coli
CC   and mammalian cells.
CC   A map is provided in this reference. [2]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pHEBo1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(vertebrate cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. EBV, oriP
FT                   3. HSV, tk promoter/#X14112
FT                   4. E. coli 1142bp, hyg gene
FT                   -> pHEBo1 6900bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI-BglII-EcoRI-ClaI-HindIII-
FT                   EcoRV-BamHI-SalI-BstEII-SmaI-SphI-SstII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   promoter        0..0
FT                   /note="PRO HSV thymidine kinase tk gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT   CDS             0..0
FT                   /note="ANT vertebrate hygromycin phosphotransferase
FT                   gene; hygromycin resistance gene (hyg)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//