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ID   PHG276     preliminary; circular DNA; SYN; 4320 BP.
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AC   ATCC37619;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHG276 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHG167 from pHG163 & pHG146
RC   pHG194 from pHG165 & lambda cI857 Sam7
RC   pHG214 from pHG194
RC   pHG216 from pHG165 & pHG214
RC   pHG229 from pLG400 & pHG216
RC   pHG267 from pHG134 & pHG216
RC   pHG271 from pLG400 & pHG167
RC   pHG276 from pHG165 & pHG267
RA   Stewart G.S., Lubinsky-Mink S., Kuhn J.;
RT   "pHG276: a multiple cloning site pBR322 copy number vector expressing
RT   a functional lac alpha peptide from the bacteriophage lambda pR
RT   promoter";
RL   Plasmid 15:182-190(1986).
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CC   A 1.7 kb BamHI/HpaI fragment containing the cI gene and lambda PR was
CC   cloned into BamHI/HpaI partially digested pHG165 (ATCC 37618).
CC   Plasmid expression vector with lambda PR promoter, plasmid copy number
CC   controlled by rop(rom), and cI857 for temperature regulation of lac
CC   and insert expression.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.4; HpaI--3.0, 1.3. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pHG276)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM101)(E.coli)(E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI 4361bp 4360..4360
FT                   fill in
FT                   HpaI linker 6bp gttaac
FT                   -> pHG134 4364bp
FT                   1. pHG165 BamHI 3370bp, pUC8 241..241
FT                   \ pUC8 BamHI = 241
FT                   2. lambda cI857 Sam7 BglII-BglII 2392bp 35712..38104,
FT                   \ cI gene/pR
FT                   \ BglII = 416 22426 35712 38104 38755 38815
FT                   -> pHG194 5760bp
FT                   1. pHG194 AvaI 5760bp, pUC8 236..236
FT                   S1 nuclease, removes 4bp
FT                   -> pHG214 5760bp
FT                   1. pHG165 remove small PvuII-EcoRI 176bp 377..553,
FT                   \ 3000bp
FT                   \ pUC8 55..231 or 231..356
FT                   2. pHG214 EcoRI-FnuDII 1647bp, lambda cI gene/pR
FT                   \ pUC8 EcoRI = 231/231..239..241..-..241
FT                   -> pHG216 4700bp
FT                   1. pHG134 remove ClaI-BamHI 25..376 351bp, 4010bp
FT                   2. pHG216 ClaI-BamHI 1155bp, lambda cI gene/pR
FT                   -> pHG267 5100bp
FT                   1. pHG165 remove BamHI-HpaI 191bp 241..432, 3150bp
FT                   \ pUC8 BamHI = 241
FT                   2. pHG267 BamHI-HpaI 1167bp, lambda cI gene/pR
FT                   \ HpaI at ends of lacZ
FT                   -> pHG276 4320bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI-BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PR"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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