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ID   PHG44      preliminary; circular DNA; SYN; 4600 BP.
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AC   ATCC39806;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHG44 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRO12 from pBR322 & lambda, pL
RC   pHG44 [similar to pAL500] from pRO12 & pPS1, rrnB T1T2
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,742,004 dated May 3, 1988.
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CC   Restriction digests of the clone give the following sizes (kb):
CC   HdeI--4.6; HindIII--3.4, 1.2; PstI--2.45, 2.1; EcoRI--4.6.
CC   (ATCC staff)
CC   A HindIII fragment from pPS1 (ATCC 39807) containing the terminator
CC   T1T2 was inserted in the HindIII site of pRO 12 to produce pHG44.
CC   Similar to pAL500 (ATCC 39782). Construct has a promoter and
CC   terminator.
CC   Expresses Met-Asp-Gln-bovine growth hormone.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pHG44)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, rrnB T1T2
FT                   -> pPS1
FT                   1. pBR322 4361bp
FT                   2. lambda, pL
FT                   -> pRO12
FT                   1. pRO12 HindIII 4400bp
FT                   2. pPS1 HindIII-HindIII 200bp, E. coli rrnB T1T2
FT                   -> pHG44 4600bp [similar to pAL500]"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-NdeI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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