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ID   PHHLUC     preliminary; circular DNA; SYN; 6200 BP.
AC   M15077; ATCC37581;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pHHluc - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   deltaBam from pSV232AL-Adelta5'
RC   pSluc, pSluc2 from deltaBam & BamHI linker
RC   pXPB from pSluc2 & linker
RC   pXPBg from pXPB
RC   pXP1, pXP2 from pXPBg
RC   pSluc2A from deltaBam
RC   pHH from MMTV
RC   pHHLUC from pXP2 & pHH
RC   pMLUC from pHHLUC
RC   pXPL from pSluc
RC   pT109luc, pT81luc from pXP2
RA   Nordeen S.K.;
RT   "Luciferase reporter gene vectors for analysis of promoters and
RT   enhancers";
RL   Biotechniques 6:454-458(1988).
CC   One of a series of luciferase vectors (ATCC 37574-37584) for assaying
CC   promoter activity in mammalian cells. [1]
CC   The insert contains bp -223 to +105 of the MMTV promoter with a
CCC  functional HRE. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.6; HindIII--6.6; SalI--6.6; matches depositor's DNA. (ATCC
CC   staff)
CC   This is a derivative of plasmids patented by the University of
CC   California and is not to be used for commercial purposes without prior
CC   arrangement with the University of California. (personal
CC   communication)
CC   There is cryptic promoter activity which may be more readily observed
CC   with weak promoters. Be careful to do all appropriate controls and map
CC   the transcripts. (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pHHluc)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. MMTV C3H HaeIII-HpaII 327bp -225..+102
FT                   -> pHH
FT                   1. pXP2 BglII 5900bp
FT                   2. pHH BamHI-BamHI 327bp, MMTV promoter
FT                   -> pHHluc 6200bp"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-HindIII-SalI-SmaI-KpnI-SstI-XhoI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-HindIII-SalI-SmaI-KpnI-
FT                   SstI-XhoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO MMTV"
FT   CDS             0..0
FT                   /note="GEN firefly luciferase gene (luxA);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;