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ID   PHISGAL    preliminary; circular DNA; SYN; 3910 BP.
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AC   ATCC87093;
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DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid pHisGal - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHisGal from pBR322 & his & GAL4
RA   Schmitz M.L., Baeuerle P.A.;
RT   "A vector, pHisGal, allowing bacterial production of proteins
RT   fused to a hexahistidine-tagged Gal4 DNA-binding domain";
RL   Biotechniques 17:714-718(1994).
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CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--4.0; PvuI--4.0; XbaI--4.0. (ATCC staff)
CC   Expression vector for production of Gal4 fusion proteins, allowing
CC   protein purification by affinity chromatography through a nickel
CC   nitrilotriacetic acid (Ni-NTA) column. For expression of cloned
CC   inserts, the medium should contain 10 uM zinc chloride and expression
CC   can be induced using IPTG. [1]
CC   Plasmid contains the following restriction sites
CC   (approximate kb from the BamHI site):  XbaI--1.51; PvuI--3.15. [1]
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Schmitz M.K.
CC   NM (pHisGal)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli XL1-Blue)
CC   CP ()
CC   FN (cloning)(fusion)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. histidine repeat
FT                   3. yeast, GAL4
FT                   -> pHisGal 3910bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast CEN4"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   promoter        0..0
FT                   /note="PRO yeast GAL4 DNA-binding domain (aa 1-147)"
FT   misc_feature    0..0
FT                   /note="hexahistidine sequence, epitope"
FT   misc_binding    0..0
FT                   /note="MCS SmaI-NarI-NaeI-ClaI-EcoRV-NruI-HindIII"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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