ID   PHPK255    preliminary; circular DNA; SYN; 1455 BP.
XX
AC   S84689;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   E. coli plasmid vector pHPK255 - replication-initiation - incomplete.
XX
KW   cloning vector.
XX
OS   Helicobacter pylori
OC   Unclassified.
XX
RN   [1]
RC   pHPK255 from H. pylori
RC   pIHPK255 from pHPK255 & pUC18
RC   pLB4 from Lactobacillus plantarum
RA   Kleanthous H., Clayton C.L., Tabaqchali S.;
RT   "Characterization of a plasmid from Helicobacter pylori encoding a
RT   replication protein common to plasmids in gram-positive bacteria";
RL   Mol. Microbiol. 5:2377-2389(1991).
XX
CC   NM (pHPK255)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Helicobacter pylori)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Helicobacter pylori)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
XX
FT   misc_feature    0..0
FT                   /note="1. H. pylori
FT                   -> pHPK255"
FT   CDS             complement(714..1361)
FT                   /note="GEN Helicobacter replication-initiation protein
FT                   homolog; for the protein sequence (NCBI gibbsq 84699);
FT                   this sequence comes from fig 2. [1]"
FT   rep_origin      0..0
FT                   /note="ORI Helicobacter pylori"
XX
SQ   Sequence 1455 BP; 436 A; 254 C; 281 G; 484 T; 0 other;
     cttcacacca ctctacaacc aacgcaaaac aaaaaaccga ctattgatac tacctacaag
     ctaaaaatcg cttaaaacga aaatatgagc gaaaaaagct atattccact cacacctaga
     agctaatcag ttagcaaatc tcacgagggt agccaataaa agcgcatgta acctcggcta
     tcaatctcac caaaaagcaa aaaaaagggg caaaaatttg ggggtgaaaa tggggggata
     taaagggcga aaattttttg aattttcgcc ctactacgac tatatccccc catgtgtaag
     ctacaagcta cgaccgttat tttttaccca tgaactgcac tcaataatga gaatgaaaag
     gggggctttt ccccccttaa aacattctca ttataggagg gttttagggg gaacaactaa
     caagctattt tgtaatcttg tattacaaaa tatatgcttg cccacaacaa aaaagcagag
     cgaacgctag tgagcaaaaa caagcggttt tgcacagaga aaatgagaat aggaaaaatt
     aagggggtgt aaaaaaaagt gttctgttta cttttcttag ataataatca ttattatttg
     gtcgtagcaa gcattttgct acaaattaac tttttgctac gactttttaa agtgaaaatt
     cactgatttt ttagcgattt tagagctaaa aaatcagaca tttttgctta tgattatctg
     cggtgttttt ctcgctgtga agtgataagt gctgtaatgt gtcttgtgta tttgtggcgt
     aataatttag cccattttgg ctgattatcc atagcccaaa aaaacaagtc agaatattca
     gtaatgcctt gttcttggat aaaaaactca atatcactaa tgagctgtag ttctagctct
     ttaagttctt ttttgctttt aaagtcgtca atatcaaagc ctttaaagtg ctgtatctcg
     cttttgtcgt attggtattt gtggctgtct ttggggtttg agtgggtaaa atactcataa
     gcccctttca ctctttcgtt gttggtgtaa gtaatcgttt tttcgttttg gttaatcaac
     ttcaacaact tcttaaccgc tgcgggtcgt tgctttttgt caaatacgat tatcgcatgg
     aagtgcggtt tcttgtggct tccgtcttcg ttcaagtctt tatcgtgtag tggtgataat
     gcccatgagc cgtcaaaatt ttcattaaga atttcaacaa aatttggggt agcactttct
     gggtagatga taaatccaaa cgctttgtag ggggcgtttt cttggttgta tttgtcatgt
     tttgagtgcg tttcagcact atttggctgt ttttttggca taatagtgtc ctttcggttt
     taattttttt tcggtgtttg aggttggatt tttcaaacac cgagcttaat tctacaacaa
     aatttcaaaa acaag
//