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ID   PHPS9R     preliminary; circular DNA; SYN; 4100 BP.
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AC   ATCC37818;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/E.coli plasmid vector pHPS9R - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHP9 from pHP3
RC   pHP9R from pHP9
RC   pHP9D from pHP9
RC   pHP9D0 from pHP9
RC   pHP9R1 from pHP9D0
RC   pHP9D1100 from pHP9D0 & E.coli DNA
RC   pHP9D1600 from pHP9D0 & E.coli DNA
RC   pHP9D3000 from pHP9D0 & E.coli DNA
RC   pHP9D4000 from pHP9D0 & E.coli DNA
RC   pHP9R2 from pHP9R1
RC   pHP9D1 from pHP9D0
RC   pHPS9R from pHPS9
RA   Haima P., Bron S., Venema G.;
RT   "Novel plasmid marker rescue transformation system for molecular
RT   cloning in Bacillus subtilis enabling direct selection of
RT   recombinants";
RL   Mol. Gen. Genet. 223:185-191(1990).
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CC   Recommended for pHPS9 (ATCC 37816, 37817) to permit
CC   alpha-complementation and plasmid marker rescue transformation for
CC   transformation of competent cells with plasmid monomers.
CC   pHPS9R serves as the helper plasmid.
CC   Differs from pHPS9 (ATCC 37816, 37817) by partial removal of
CC   chloramphenicol resistance sequence (downstream of the NcoI site) and
CC   removal of the P59 promoter and sequences encoding the
CC   cat86::lacZalpha fusion.
CC   Plasmid was not verified by restriction analysis.  Strain was checked
CC   for the following phenotypes: showed growth on LB + erythromycin
CC   (150 ug/ml), white colonies on Xgal +IPTG, white colonies on Xgal +
CC   IPTG + erythromycin (150 ug/ml). (ATCC staff)
CC   Medium is -1 1065 plus erythromycin (150 ug/ml).
CC   NM (pHPS9R)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis 6GM15)(Bacillus subtilis)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHPS9 remove 1500bp, cat-86 gene/lacZalpha
FT                   \ 4100bp
FT                   -> pHPS9R 4100bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pTA1060"
FT   CDS             0..0
FT                   /note="ANT E. coli erythromycin resistance gene (ery)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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