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ID   PHS1       preliminary; circular DNA; SYN; 260 BP.
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AC   M10912; M10913;
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DT   15-JUN-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHS1 - incomplete, integration site for Tn3#2.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   from pHS1, IS102/tet gene
RA   Hashimoto-Gotoh T., Sekiguchi M.;
RT   "Mutations to temperature sensitivity in R plasmid pSC101";
RL   J. Bacteriol. 131:405-412(1977).
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RN   [2]
RC   pHS1, pHS2, pHS4, pHS6, pHS7, pHS17 from pSC101
RC   pHS3 from pSC101
RC   pHS5, pHS8, pHS16 from pSC101
RC   [pHSG1 from pSC101]
RA   Hashimoto T., Sekiguchi M.;
RT   "Isolation of temperature-sensitive mutants of R plasmid by in
RT   vitro mutagenesis with hydroxlamine";
RL   J. Bacteriol. 127:1561-1563(1976).
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CC   NCBI gi: 209011 & 209012
CC   NM (pHS1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSC101 9263bp
FT                   -> pHS1"
FT   misc_recomb     44..48
FT                   /note="SIT pHS1 target site for recombination; first"
FT   misc_recomb     33..37
FT                   /note="SIT pHS1 target site for recombination; second"
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SQ   Sequence 260 BP; 33 A; 50 C; 53 G; 35 T; 89 other;
     ctgtcgctga ataacgtcac ggactcagaa gccttcccgg gccttatccg gcagactcac
     agaaaaatca gggcagccgc ggcagacggg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     ccggcagact ggtacagagc atggtcacgg gctttacggg cggctctggc ttcggctcgc
     ttttctgcct gtatcaggtt
//