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ID   PHSG250    preliminary; circular DNA; SYN; 1700 BP.
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AC   ATCC37299;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli cosmid vector pHSG250 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHSG306 from ColE1 & Tn5
RC   pHSG415s from pHSG415
RC   pHSG418 from pHSG415s
RC   pHSG425 from pHSG418
RC   pHSG429 from pHSG425 & pHSG422
RC   pHSG124 from ColE1 & Tn3 & pHSG1
RC   pHSG402 from pHSG124
RC   pHSG231 from pHSG402
RC   pHSG232 from pHSG231
RC   pHSG255 from pHSG232 & pHSG429
RC   pHSG250 from pHSG255
RC   pHSG262 from pHSG255
RC   pHSG415h from pHSG415s
RC   pHSG413h from pHSG415h
RC   pHSG439 from pHSG413h & pHSG262
RC   pBRG418 from pBR322 & pAG60, Tn5 kan/HSV tk gene promoter/polyA
RC   pHSG272 or pHS272 from pBRG418 & pHSG262
RC   pHSG274 from pBRG418 & pHSG255 & pUC8
RC   pHSG591 from pNO1523, lambda cos
RC   pHSG660 from pNO1523
RA   Brady G., Jantzen H.M., Bernard H.U., Brown R., Schutz G.,
RA   Hashimoto-Gotoh T.;
RT   "New cosmid vectors developed for eukaryotic DNA cloning";
RL   Gene 27:223-232(1984).
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RN   [2]
RC   pDMS630 or 6-30 from ColE1 & Tn3
RA   Inselburg J.;
RT   "Isolation, mapping, and exanmination of effects of TnA insertions
RT   in ColE1 plasmids";
RL   J. Bacteriol. 129:482-491(1977).
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RN   [3]
RC   pSC134 from pSC101 & ColE1
RA   Timmis K., Cabello F., Cohen S.N.;
RT   "Utilization of two distinct modes of replication by a hybrid
RT   plasmid constructed in vitro from separate replicons";
RL   Proc. Natl. Acad. Sci. U.S.A. 71:4556-4560(1974).
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RN   [4]
RC   ColE1-Tn5 from ColE1 & Tn5
RA   Berg D.E.;
RT   "Insertion and excision of the transposable kanamycin resistance
RT   determinant Tn5";
RL   DNA Insertion Element, Plasmids, and Episomes 0:205-212(1977).
RL   Bukhari A.I., Shapiro J.A., Adhya S.L.;
RL   Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
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CC   A cosmid vector suitable for cloning large DNA fragments.
CC   Medium is 1065 LB medium.
CC   NM (pHSG250)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli SK1592)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSC101 9263bp, ori/tet gene
FT                   mutagenesis
FT                   -> pHSG1
FT                   1. E. coli
FT                   -> R1
FT                   1. R1
FT                   -> R1drd-19
FT                   1. ColE1 6646bp
FT                   transposition
FT                   2. R1drd-19, Tn3 amp gene
FT                   -> pDMS630
FT                   1. pHSG1 EcoRI 9263bp 9262..9262
FT                   2. pDMS630 EcoRI 6646bp 6645..6645
FT                   -> pHSG124 15909bp
FT                   1. pHSG124 remove BamHI-BamHI, pSC101 ori
FT                   \ pSC101 376 to R1drd-19 Tn3 amp gene
FT                   -> pHSG401
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   -> pSC105 16163bp
FT                   1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. pVH51 EcoRI 3847bp 3846..3846
FT                   -> pML21 10747bp
FT                   1. pHSG401
FT                   2. pML21 10747bp
FT                   -> pHSG408
FT                   1. pHSG408
FT                   -> pHSG410
FT                   1. pHSG410
FT                   -> pHSG412
FT                   1. pHSG412
FT                   2. pACYC184 4245bp
FT                   -> pHSG415
FT                   1. pHSG415
FT                   -> pHSG415s
FT                   1. pHSG415s
FT                   -> pHSG418
FT                   1. pHSG418 remove small PstI-PstI
FT                   -> pHSG425
FT                   1. pHSG415
FT                   2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT                   -> pHSG422
FT                   1. pHSG425 BstEII
FT                   2. pHSG422 BstEII-BstEII, amp gene/lambda cos
FT                   -> pHSG429
FT                   1. pHSG124 remove small BamHI-BamHI
FT                   -> pHSG402 [unique EcoRI, BamHI]
FT                   1. pHSG402 remove small HaeII-HaeII
FT                   -> pHSG231 [unique EcoRI, BamHI]
FT                   1. pHSG231 EcoRI
FT                   2. pivx EcoRI-EcoRI, MCS
FT                   EcoRI-ClaI-HindIII-XbaI-BglII-PstI-BamHI-EcoRI
FT                   -> plasmid
FT                   1. plasmid remove small BamHI-BamHI, MCS
FT                   -> pHSG232
FT                   1. pHSG232 BamHI
FT                   T4 DNA polymerase
FT                   HaeII-HaeII
FT                   2. pHSG429 HaeII-PvuII, kan gene/lambda cos
FT                   -> pHSG255 [unique EcoRI, BamHI, HincII]
FT                   1. pHSG255 HaeII-HaeII 1430bp, kan gene/#V00359
FT                   -> pHSG250 1700bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-HincII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli colicin E1 immunity gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
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