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ID   PHSG274    preliminary; circular DNA; SYN; 3600 BP.
AC   ATCC37301;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli cosmid vector pHSG274 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pHSG306 from ColE1 & Tn5
RC   pHSG415s from pHSG415
RC   pHSG418 from pHSG415s
RC   pHSG425 from pHSG418
RC   pHSG429 from pHSG425 & pHSG422
RC   pHSG124 from ColE1 & Tn3 & pHSG1
RC   pHSG402 from pHSG124
RC   pHSG231 from pHSG402
RC   pHSG232 from pHSG231
RC   pHSG255 from pHSG232 & pHSG429
RC   pHSG250 from pHSG255
RC   pHSG262 from pHSG255
RC   pHSG415h from pHSG415s
RC   pHSG413h from pHSG415h
RC   pHSG439 from pHSG413h & pHSG262
RC   pBRG418 from pBR322 & pAG60, Tn5 kan/HSV tk gene promoter/polyA
RC   pHSG272 or pHS272 from pBRG418 & pHSG262
RC   pHSG274 from pBRG418 & pHSG255 & pUC8
RC   pHSG591 from pNO1523, lambda cos
RC   pHSG429 from kan gene
RC   pHSG660 from pNO1523
RA   Brady G., Jantzen H.M., Bernard H.U., Brown R., Schutz G.,
RA   Hashimoto-Gotoh T.;
RT   "New cosmid vectors developed for eukaryotic DNA cloning";
RL   Gene 27:223-232(1984).
CC   A cosmid vector selectable in both prokaryotic and eukaryotic cells.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pHSG274)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli SK2247)(broad host range)(E.coli)(E.coli SK1592)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pSC101 9263bp, ori/tet gene
FT                   mutagenesis
FT                   -> pHSG1 9263bp [temperature sensitive, tet]
FT                   1. R1, Tn3 amp gene
FT                   -> R1drd-19
FT                   1. ColE1 6646bp
FT                   transposition
FT                   2. R1drd-19, Tn3 amp gene
FT                   -> pDMS630
FT                   1. pHSG1 EcoRI 9263bp 9262..9262
FT                   2. pDMS630 EcoRI 6646bp 6645..6645
FT                   -> pHSG124 15909bp
FT                   1. pHSG415
FT                   -> pHSG415s
FT                   1. pHSG415s
FT                   -> pHSG418
FT                   1. pHSG418 remove small PstI-PstI
FT                   -> pHSG425
FT                   1. pHSG415
FT                   2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT                   -> pHSG422
FT                   1. pHSG425 BstEII
FT                   2. pHSG422 BstEII-BstEII, amp gene/lambda cos
FT                   -> pHSG429
FT                   1. pHSG124 remove small BamHI-BamHI
FT                   -> pHSG402 [unique EcoRI, BamHI]
FT                   1. pHSG402 remove small HaeII-HaeII
FT                   -> pHSG231 [unique EcoRI, BamHI]
FT                   1. pHSG231 EcoRI
FT                   2. piVX EcoRI-EcoRI, MCS
FT                   EcoRI-ClaI-HindIII-XbaI-BglII-PstI-BamHI-EcoRI
FT                   -> plasmid
FT                   1. plasmid remove small BamHI-BamHI, MCS
FT                   -> pHSG232
FT                   1. pHSG232 BamHI
FT                   T4 DNA polymerase
FT                   HaeII-HaeII
FT                   2. pHSG429 HaeII-PvuII, kan gene/lambda cos
FT                   -> pHSG255 [unique EcoRI, BamHI, HincII]
FT                   1. Tn5 BglII-BamHI 1541bp 1516..3057, kan/neo gene
FT                   2. HSV, tk gene promoter/polyA/#X14112
FT                   -> pAG60
FT                   1. pBR322 HindIII 4361bp 30..30, P1 promoter
FT                   2. pAG60 PvuII-PvuII, Tn5 kan/HSV tk gene prom/polyA
FT                   HindIII linker 6bp aagctt:HindIII linker 6bp aagctt
FT                   -> pBRG418 [unique HindIII]
FT                   1. pBRG418 BamHI-ClaI, Tn5 kan gene
FT                   fill in:
FT                   2. pHSG255 ClaI-XhoI
FT                   :fill in
FT                   -> plasmid
FT                   1. plasmid remove RsaI-RsaI, ColE1/kan gene/pBR322
FT                   -> plasmid2
FT                   1. plasmid2 remove ClaI-HaeII, kan gene
FT                   fill in:fill in
FT                   -> plasmid3
FT                   1. plasmid3 remove HincII-BamHI, [gatcctgctttttgtt]
FT                   2. pUC8 BamHI-HincII 8bp 241..249, 11bp gatccgtcgac
FT                   -> pHSG274 3600bp [unique SalI]"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-BamHI-SalI-SmaI-PvuII-
FT                   BglII-HincII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT broad host range kanamycin resistance gene
FT                   (kan)"
FT   CDS             0..0
FT                   /note="ANT broad host range G418 resistance gene
FT                   (G418)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;