back Return to this vector's summary.
ID   PHT1030    preliminary; circular DNA; SYN; 2872 BP.
XX
AC   X59245; S80845;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   Bacillus plasmid vector pHT1030 - incomplete - spbB gene & ORF2.
XX
KW   cloning vector.
XX
OS   Bacillus thuringiensis strain LM2
OC   Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
OC   Bacillaceae; Bacillus.
XX
RN   [1]
RC   pHT1030
RA   Lereclus D.;
RT   ;
RL   Submitted (28-APR-1991) to GenBank by:
RL   Lereclus D., Institut Pasteur, 25 Rue du Docteur Roux,
RL   Paris Cedex 15, 75724, FRANCE.
XX
RN   [2]
RC   pHT3100 series from pUC18 & pHT1030
RC   pHT181 from pUC18 & ery gene
RC   [pBC16delta1 from pBC16]
RC   pHT1618 from pUC18 & pBC16delta1
RC   pHT1600 series from pHT1618
RA   Lereclus D., Arantes O.;
RT   "spbA locus ensures the segregational stability of pTH1030, a novel
RT   type of gram-positive replicon";
RL   Mol. Microbiol. 6:35-46(1992).
XX
RN   [3]
RC   pHT1000 from Bacillus thuringiensis
RC   pHT1030 from Bacillus thuringiensis
RC   pHT1001 from pHT1000 & pJH101
RC   pHT1002 from pHT1000 & pJH101
RC   pHT1004 from pHT1000
RC   pHT1005 from pHT1000 & pJH101
RC   pHT1031 from pHT1030 & pJH101
RC   pHT1032 from pHT1030 & pJH101
RC   pHT1035 from pHT1030 & pJH101
RA   Lereclus D., Guo S., Sanchis V., Lecadet M.M.;
RT   "Characterization of two Bacillus thuringiensis plasmids whose
RT   replication is thermosensitive in B. subtilis";
RL   FEMS Microbiol. Lett. 49:417-422(1988).
XX
RN   [4]
RC   pHT3101 from pUC18 & pUC18-ery & pHT1030
RC   pHT407 from pUC18 & Bacillus delta-endotoxin gene
RC   pHT408 from pHT407 & pHT3101
RC   pHT409, pHT410 from pHT407 & pHT3101
RA   Lereclus D., Arantes O., Chaufaux J., Lecadet M.M.;
RT   "Transformation and expression of a cloned delta-endotoxin gene
RT   in Bacillus thuringiensis";
RL   FEMS Microbiol. Lett. 60:211-218(1989).
XX
RN   [5]
RC   pUC18-ery from pUC18 & ery gene
RA   Trieu-Cuot P.;
RT   ;
RL   Unpublished (1989).
XX
CC   spbB gene & ORF2 is 2872 bp.
CC   NM (pHT1030)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Bacillus thuringiensis)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Bacillus thuringiensis)(pUC18)(erythromycin)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Bacillus thuringiensis
FT                   -> pHT1030"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pHT1030"
FT   -35_signal      75..80
FT                   /note="S35 Bacillus thuringiensis spbB gene"
FT   -10_signal      98..103
FT                   /note="S10 Bacillus thuringiensis spbB gene"
FT   repeat_unit     355..424
FT                   /note="RPT rpt_type=INVERTED"
FT   CDS             2521..>2872
FT                   /note="GEN Bacillus orf2"
FT   CDS             complement(1792..2193)
FT                   /note="GEN Bacillus thuringiensis spbB gene; orf1"
XX
SQ   Sequence 2872 BP; 925 A; 475 C; 587 G; 885 T; 0 other;
     ccatcctcca aagttggaga gtgagtttta tgtcgcaaat attaatgttt ctggtgaacc
     ttatcaaatt ttcgttgatt taatagaaac atagcggtaa aattagcagt aacttaatag
     aacggaaatg aaaaaagcca ctctcatatg ctattggcta ccaaccttta gcgagaatga
     cttaatcctg tacagccata caggacttcg acttataaga ggcgccaacc tcaaataagt
     tatttgcctt gttttcgcga acaaggctta ttagatacac ctattgtacc gttactctac
     gaatatttca actagtaatt actagcattg tcatatacat aataaaacgg atataaaagg
     gcgttttcta tacctagaag tcttgtaaat gtacagggcg tttagatata gagaacgccc
     tttttgtgtt ccgttccagt ggaagctacc actttaaaaa gatggtctag tgtagccaat
     gcaggagagt acactcggat atcagttgtc gttgcattca actgtctgac gtaagcgagg
     taaaggacac aagccttgca taaaacaagc ctacgggatg taaatcctaa taatgatgat
     aaccaagacg ttagcggcaa aaagtgttgg gggttcaaaa taagacatga ttgtgcgact
     ggagttaaac agttactcgt aagcggcgat catgacactg attcacggct attcttgtac
     aagctttatt acaaggatat gcgggttata tagcgaatca cccgaaaggg aacggtgttg
     ggcgtgagaa acgcaccgta cggcgcaata caatgccaat aagctatata cggacggtat
     agtagttttg taagctataa ccgtttgtcg tcaatgcaac caatctcaat tcgagacctc
     ggcatctaag ccagtacgaa tgagtgggcg ttttaacctc gtaaattttc aacaggggtt
     actatgccca aaactacatt cagatttcct aacaaactcg ccagtatgaa aaccttaaga
     ccttaaagtc aagggatttg aaggatttta acctcgatta gcaaaaaatg tagagtactg
     aagcaactac cattaactaa gatagtgggg gattgaggaa gaatccagag ctgtttaaat
     caagtgaaag acaagatgaa attaaaagaa tagtgaaaga taggggagtg gttctctatg
     agaaaggaaa tggctagaga acaaaggcag cggtttattg atctattgtt agactttatg
     gtaaagaatc ctcatttatt tgttaatggt acagaggatg aaagtaataa tgttgttaca
     aaatgtaata gtgatattaa agaggttgcg gagtcatatt taactctttt atagtgagag
     ggttaaaact aattaatatg tattaaggcc caatgttgga attattgtat ttcactaggc
     aacctactta ctaaaagtaa gattatccat tagtggatgt tataatattg ggttttttaa
     cacaataatc atcgcctttc ggtgtcgttt gatagaaaag taaccattag cgatgaaaaa
     gtcaatataa aaagccatcc gtaaaaaacg gatggcttac cgtacatagg atcgttggta
     gggcggcgta tcctacatct ctggtaactt acctagccaa tcaaatgctt gagaacggcg
     gttagataag cgcgtgggga acctttccca cctcaaagat cctatatcat tattatgtta
     ctttctacag gtagtatacc atgttcttat attttagtaa actccccgtt agcttaacag
     gtctttgtaa gcaattaaac gtccactatt caatcgtctt tggattttcg caggaccgtt
     ttttagatcg aacatagttg ataagaacaa ataaccgctt gggtccaact ttatagcaat
     tagtatatgg tcatttaaaa tctttaccaa ttcaacgcta ttaggttctt taggattttg
     cccgacatag tcggggtgtt caacgatatc ttttatgtgc gatgaatatt tttcataaat
     accaggatgt tgtttcttta cgtgctttat aaatccggga aacattttta catcgttaga
     agtgcaagtc aagttatatg tatctataat gatttgtgga agttttgcca caacagttgg
     tttatttaca atcttttttt tattagccgt caaatttctc cctcatctcg tctctttata
     tctttatttt atcataaagg agtatttgaa ccgtcgcgcg ggacaggttt atgataggga
     tattttattg aataattgat ggtataaggg actttcatgc ttggaaagtg gggattatga
     attagatgct tgtccacaat atgttccaat gtaattaaaa tttatgttcc caccttgacc
     aaacatcacg tccatactta aatcgtccct cctttaatag gtaaaatatt aatttacctt
     aataaaaaaa taatggataa tagtattcgt ctgaatttat ataatcaggg ggaactattg
     atgctgggga tactatttac agcggcgcca tctactgatg tcgtaaagga tttgcaagat
     aaagttatat cattgcagga tcatgaggta gcgtttttga acaccacgat atctaatatg
     ttaacagcag taggtattgg agtggcaatt ataacggcgg tttttacagc agcgtttgct
     tatgttacat attctaataa gcgtgctaaa aagaatatgg acgaggctag tagaaaatta
     gaagaagcag aaagtaaagt ttctgtgcta gaggagaaaa gcgctcaatt ggagaggaag
     attcttgaag ctgaacaact cttagctgat gccaattcta tttctaatgt gg
//