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ID   PHUB2      preliminary; circular DNA; SYN; 7600 BP.
AC   ATCC37091;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pHUB2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pER481, pER482 from lambda trp48 & pCR1
RC   pMK2004lambdatrp43 from pMK2004 & lambda trp43
RC   pMK2004lambdatrp48 from pMK2004 & lambda trp48
RC   pMK2004lambdatrp43trpA from pMK2004lambdatrp43 & trpA gene
RC   pMK2004lambdatrp48trpA from pMK2004lambdatrp48 & trpA gene
RC   pHUB2 from pMK2004lambdatrp43, lambda pL prom
RC   pHUB4 from pMK2004lambdatrp43
RC   pHUB2trpA from pHUB2 & trpA gene
RC   pRK248cIts from pRK248 & pHUB2trpA & pER482, tet gene/cIts gene
RA   Bernard H.U., Remaut E., Hershfield M.V., Das H.K., Helinski D.R.,
RA   Yanofsky C., Franklin N.;
RT   "Construction of plasmid cloning vehicles that promote gene
RT   expression from the bacteriophage lambda pL promoter";
RL   Gene 5:59-76(1979).
RN   [2]
RC   pDF21 from mini-F & E. coli trpED genes
RC   pCR1 from pML2
RC   pVH151 from ColE1 & trpED genes
RC   pVH153 from ColE1 & trpED genes
RA   Armstrong K.A., Hershfield M.V., Helinski D.R.;
RT   "Gene cloning and containment properties of plasmid ColE1 and its
RT   derivatives";
RL   Science 196:172-174(1977).
RN   [3]
RC   pMK2004 from pBR322 & kan gene
RA   Kahn M.;
RT   ;
RL   Unpublished (1979).
RN   [4]
RC   lambda trp9, lambda trp43, lambda trp46, lambda trp48 from lambda h80
RC   lambda trp61 from lambda h80
RA   Franklin N.C.;
RT   "Altered reading of genetic signals fused to the N-operon of
RT   bacteriophage lambda: genetic evidence for modification of
RT   polymerase by the protein product of the N gene";
RL   J. Mol. Biol. 89:33-48(1974).
RN   [5]
RC   pRK248 from RK2 & tet gene
RA   Meyer R., Helinski D.R.;
RT   ;
RL   Unpublished (1979).
RN   [6]
RC   pJC307 from ColE1
RA   Collins J., Yanofsky S., Helinski D.R.;
RT   "Altered DNA-protein relaxation complex in a replication mutant of
RT   plasmid ColE1";
RL   Mol. Gen. Genet. 167:21-28(1978).
CC   Expression vector under control of lambdaPL and temperature sensitive
CC   lambda repressor.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pHUB2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Enzobiochem)(ATCC)
CC   HO (E.coli JMB9[lambda])(E.coli JMB9 [pRK248cIts])
CC   HO (E.coli K-12 deltaH1)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda h80
FT                   1. lambda h80, N operon/pL
FT                   2. E. coli trp operon
FT                   -> lambda trp43
FT                   1. pBR322 4361bp
FT                   2. kan gene
FT                   -> pMK2004
FT                   1. pMK2004 remove PstI-EcoRI 4400bp, amp gene
FT                   2. lambda trp43 PstI-EcoRI 10000bp, pL/trpDCB
FT                   -> pMK2004lambdatrp43 [1 EcoRI 3' to pL]
FT                   1. pMK2004lambdatrp43 remove HpaI-HpaI 7900bp, trpDCB
FT                   -> pHUB2 7600bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI-EcoRI-BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda pL"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;