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ID   PHV100     preliminary; circular DNA; SYN; 5200 BP.
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AC   M28701;
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DT   15-DEC-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHV100 - incomplete, lacI/lacZ/luxA genes/MCS.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-396
RC   pHV100 from pUC18 & pMT1
RC   pHV200 from pHV100
RC   pUCT1T2, pUCT2T1 from pUC19 & pKK65-10, E. coli rrnB T1T2 terminator
RC   pHVT1T2 from pUCT1T2 & pHV100
RC   pHVT2T1 from pUCT2T1 & pHV100
RC   pHVtR1 from pHV100 & lambda, tR1 terminator
RA   Peabody D.S., Andrews C.L., Escudero K.W., Devine J.H.,
RA   Baldwin T.O., Bear D.G.;
RT   "A plasmid vector and quantitative techniques for the study of
RT   transcription termination in Escherichia coli using bacterial
RT   luciferase";
RL   Gene 75:289-296(1989).
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RN   [2]
RC   pTB7 from pBR322 & luxA/luxB
RC   pMT1 from pBR322 & luxA/luxB
RA   Baldwin T.O., Berends T., Bunch T.A., Holzman T.F., Raush S.K.,
RA   Shamansky L., Treat M.L., Ziegler M.M.;
RT   "Cloning of the luciferase structural genes from Vibrio harveyi
RT   and expression of bioluminescence in E. coli";
RL   Biochemistry 23:3663-3667(1984).
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CC   NCBI gi: 207740
CC   NM (pHV100)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (E.coli)(V.harveyi)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. E. coli 1200bp, luxA/luxB genes
FT                   -> pMT1 5600bp
FT                   1. pUC18 TaqI-SalI 2685bp 419..2686..418
FT                   2. pMT1 NdeI-ClaI 2087bp 2299..4361..25, ori/amp gene
FT                   3. pMT1 SalI-NdeI 500bp, luxA/luxB genes
FT                   \ pBR322 652..2299
FT                   -> pHV100 5200bp"
FT   mRNA            173..229
FT                   /note="RNA E. coli lacZ mRNA"
FT   misc_recomb     230..259
FT                   /note="E. coli DNA end/polylinker DNA start"
FT   misc_binding    259..396
FT                   /note="MCS"
FT   misc_recomb     260..>396
FT                   /note="polylinker DNA end/V.harveyi DNA start"
FT   CDS             <1..88
FT                   /note="REP E. coli lacI gene;
FT                   NCBI gi: 207741"
FT   CDS             211..226
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   partial; NCBI gi: 207742"
FT   CDS             394..>396
FT                   /note="GEN firefly luciferase gene (luxA);
FT                   reporter gene; NCBI gi: 207743"
XX
SQ   Sequence 396 BP; 109 A; 96 C; 95 G; 96 T; 0 other;
     caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     gcggataaca atttcacaca ggaaacagct atgaccatga ttacgaattc gagctcggta
     cccggggatc ctctagagtc gactttatcg agcctgattt tgaacaactc accatcgcga
     ctgtgaatga acgtcgcttg aaagcggaaa ttgaaagccg tacgccagaa atggcttagg
     tcttatcgta ataccaacaa ataaggaaat gttatg
//