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ID   PHV33      preliminary; circular DNA; SYN; 6900 BP.
AC   ATCC39217;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Bacillus/E.coli plasmid vector pHV33 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pHV22 from pHV23
RC   pHV32 from pHV14
RC   pHV33 from pHV14
RA   Primrose S.B., Ehrlich S.D.;
RT   "Isolation of plasmid deletion Mutants and study of their
RT   instability";
RL   Plasmid 6:193-201(1981).
RN   [2]
RC   pHV11 from pC194 & tet gene
RC   pHV12 from pC194 & pBR313
RC   pHV14 from pC194 & pBR322
RC   pHV15 from pC194 & pBR322
RC   pHV16 from pC194 & pWL7
RC   pHV23 from pBR322 & pHV11
RC   pFT24 from pSC101 & phage Phi-3-T, thymidylate synthetase gene
RC   pFT501 from pFT24
RC   [pHV425 from pBR322 & pHV11]
RA   Ehrlich S.D., Niaudet B., Goze A., Primrose S.B.;
RT   "Transformation of Bacillus subtilis with plasmid DNAs";
RL   Transformation 1978 0:269-277(1979).
RL   Glover S.W., Butler L.O., Cotswold Press, Oxford.
RN   [3]
RC   pHV11 from pC194 & pT127
RC   pHV12 from pC194 & pBR313
RC   pHV14, pHV15 from pC194 & pBR322
RC   pHV16 from pC194 & pWL7, amp gene/kan gene
RC   pHV18 from pHV12 & pSC105, kan gene
RA   Ehrlich S.D.;
RT   "DNA cloning in Bacillus subtilis";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:1433-1436(1978).
RN   [4]
RC   pHV600, pHV601 from pHV33 & B.amyloliquefaciens DNA
RC   from pC194 & B.amyloliquefaciens DNA
RC   pHV39 from pHV33 & pUB110
RC   pHV41 from pHV39
RA   Michel B., Palla E., Niaudet B., Erlich S.D.;
RT   "DNA cloning in Bacillus subtilis III. Efficiency of random-segment
RT   cloning and insertional inactivation vectors";
RL   Gene 12:147-154(1980).
RN   [5]
RC   pHV60 from pHV33
RC   pHV600 series from pHV60 & pUB110
RA   Michel B., Niaudet B., Ehrlich S.D.;
RT   "Intermolecular recombination during transformation of Bacillus
RT   subtilis competent cells by monomeric and dimeric plasmids";
RL   Plasmid 10:1-10(1983).
CC   This is an ustable, tetracycline resistant mutant of pHV14.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--7.9; HindIII--4.65, 3.05. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pHV33)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli RR1)(Bacillus subtilis)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pC194 HindIII, complete
FT                   2. pBR322 HindIII, complete
FT                   -> pHV14 6900bp
FT                   1. pHV14, tet gene
FT                   mutation
FT                   -> pHV33 6900bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-PstI-BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;